AP-2alpha and AP-2gamma are transcriptional targets of p53 in human breast carcinoma cells.

Oncogene
H LiFrederick E Domann

Abstract

Activating enhancer-binding protein 2alpha (AP-2alpha) and activating enhancer-binding protein 2gamma (AP-2gamma) are transcription factors that bind GC-rich consensus sequences and regulate the expression of many downstream genes. AP-2alpha and AP-2gamma interact with p53 both physically and functionally. Expression microarray results in human breast carcinoma cells with forced p53 expression revealed AP-2gamma as a putative transcriptional target of p53. To confirm and extend these findings we measured the effects of forced p53 expression in human breast carcinoma cells by real-time reverse transcription-PCR, Western blotting, electrophoretic gel mobility shift assays, promoter reporter, chromatin immunoprecipitation and chromatin accessibility assays. Wild-type p53 expression rapidly induced not only AP-2gamma but also AP-2alpha mRNA. The subsequent increase in these proteins led to increased AP-2 DNA-binding and transactivating activity. Candidate p53-binding sites were identified in the AP-2alpha and AP-2gamma promoters. p53 binding to these cis-elements in vivo was also observed, together with a relaxation of chromatin structure in these regions. Finally, expression of either AP-2alpha or gamma inhibited growth of human b...Continue Reading

References

Aug 1, 1970·The British Journal of Venereal Diseases·B M LevineC W Israel
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·S D HurstingJ M Phang
Dec 1, 1995·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·R MeierR Brignoli
Jan 1, 1997·Nature Genetics·Y X ZengW S el-Deiry
Sep 26, 1997·Nature·K PolyakB Vogelstein
Aug 26, 1998·Biochemical and Biophysical Research Communications·Y Huang, F E Domann
Nov 17, 1998·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J M KarjalainenV M Kosma
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·J YuB Vogelstein
Jun 3, 2000·Organic Letters·H ChallaS A Woski
Dec 1, 2000·Nature·B VogelsteinA J Levine
Mar 30, 2001·The Journal of Biological Chemistry·C H ZhuF E Domann
Sep 13, 2002·The Journal of Biological Chemistry·Lisa A McPhersonRonald J Weigel
Oct 19, 2002·Oncogene·Michele ModugnoSylvie Ménard
Apr 16, 2003·Differentiation; Research in Biological Diversity·Judith A West-MaysM Elizabeth Fini
Jun 6, 2003·Oncogene·Marc M OshiroBernard W Futscher
Feb 15, 1964·Nature·R M MENDENHALL, G N SUN
Apr 21, 2004·Journal of Molecular Biology·Rodolfo GhirlandoGary Felsenfeld
Jun 9, 2004·Gene Expression Patterns : GEP·A K BosserhoffR Buettner
Jun 16, 2004·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J PellikainenV-M Kosma
Nov 17, 2005·Oncogene·P R StabachR J Weigel
Dec 6, 2005·Developmental Biology·Maranke I KosterDennis R Roop
Apr 1, 1997·Cell Death and Differentiation·K PolyakB Vogelstein

❮ Previous
Next ❯

Citations

Jun 4, 2011·Genetics and Molecular Biology·Yasuhito Ohsaka, Hoyoku Nishino
May 9, 2012·Free Radical Biology & Medicine·Sanjit Kumar Dhar, Daret K St Clair
Dec 13, 2006·The Journal of Investigative Dermatology·Rose-Anne RomanoSatrajit Sinha
Dec 9, 2014·Cell Cycle·Martin FischerKurt Engeland
Nov 19, 2010·Cell Cycle·Angelo G ScibettaHelen C Hurst
Mar 25, 2008·Developmental Biology·Bing XuYu-Chung Yang
Feb 10, 2011·Endocrinology·Katarzyna BiadasiewiczMartin Knöfler
Oct 6, 2017·Oncotarget·Sonja HelbigJonathan Beesley
Nov 26, 2010·Molecular Biology Reports·Dan ZhengYumin Mao
Jul 23, 2014·International Journal of Molecular Medicine·Xiangjun MengLili Jia
Oct 13, 2017·Molecular Cancer Research : MCR·Synnøve YndestadHans Petter Eikesdal
Jul 27, 2006·Neoplasia : an International Journal for Oncology Research·Hualei LiFrederick E Domann

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.