Ap3A and Ap4A are primers for oligoadenylate synthesis catalyzed by interferon-inducible 2-5A synthetase

FEBS Letters
K TurpaevJ Justesen

Abstract

The biological role of Ap3A synthesized in cells by tryptophanyl-tRNA synthetase (WRS) is unknown. Previously we have demonstrated that the cellular level of Ap3A significantly increases after interferon treatment. Here we show that the human 46 kDa 2-5A synthetase efficiently utilizes Ap3A as a primer for oligoadenylate synthesis. The Km for Ap3A is several-fold lower than for Ap4A and 100-fold lower than for ATP. This implies that Ap3A might be a natural primer for the 2'-adenylation reaction catalysed by 2-5A synthetase. Since WRS and 2-5A synthetase are both interferon-inducible proteins, a new link between two interferon-dependent enzymes is established.

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Citations

Sep 29, 2000·Pharmacology & Therapeutics·A G McLennan
May 20, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·D Rebouillat, A G Hovanessian
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Feb 3, 1999·Annual Review of Genetics·K HuebnerC M Croce
Sep 23, 1998·FEBS Letters·O MadridM A Günther Sillero
Sep 16, 1999·FEBS Letters·K TurpaevJ Justesen
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Sep 15, 1998·Methods : a Companion to Methods in Enzymology·S N Sarkar, G C Sen
Feb 21, 2004·Biochemical and Biophysical Research Communications·Luminita H PojogaRichard H Hilderman

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