Apamin inhibits PDGF-BB-induced vascular smooth muscle cell proliferation and migration through suppressions of activated Akt and Erk signaling pathway

Vascular Pharmacology
Jung-Yeon KimKwan-Kyu Park

Abstract

The increased proliferation and migration of vascular smooth muscle cells (VSMC) are key process in the development of atherosclerosis lesions. Platelet-derived growth factor (PDGF) initiates a multitude of biological effects that contribute to VSMC proliferation and migration. Apamin, a component of bee venom, has been known to block the Ca(2+)-activated K(+) channels. However, the effects of apamin in the regulation PDGF-BB-induced VSMC proliferation and migration has not been identified. In this study, we investigate the inhibitory effect of apamin on PDGF-BB-induced VSMC proliferation and migration. Apamin suppressed the PDGF-BB-induced VSMC proliferation and migration with no apparent cytotoxic effect. In accordance with these findings, apamin induced the arrest of cell cycle progression at G0/G1 phase. Apamin also decreased the expressions of G0/G1 specific regulatory proteins including proliferating cell nuclear antigen (PCNA), cyclin D1, cyclin-dependent kinases (CDK) 4, cyclin E and CDK2, as well as increased the expression of p21(Cip1) in PDGF-BB-induced VSMC. Moreover, apamin inhibited PDGF-BB-induced phosphorylation of Akt and Erk1/2. These results suggest that apamin plays an important role in prevention of vascula...Continue Reading

References

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Citations

Nov 8, 2015·Biochemical and Biophysical Research Communications·Qi-Feng LiuBao-Ze Qu
Mar 5, 2015·Vascular Pharmacology·Chiara Ricci, Nicola Ferri
Feb 11, 2020·Clinical and Experimental Pharmacology & Physiology·Baowei SuGuang Luo
Apr 14, 2017·International Journal of Molecular Medicine·Jung-Yeon KimKwan-Kyu Park
Jan 25, 2019·Journal of Translational Medicine·Ramu AdelaSanjay K Banerjee
Jun 21, 2020·International Journal of Molecular Sciences·Jihyun ParkKwan-Kyu Park
Aug 23, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rim WehbeZiad Fajloun
Jan 6, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anna Kurek-GóreckaPaweł Olczyk
May 26, 2021·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Mi-Gyeong GwonKwan-Kyu Park
May 2, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xunzhong DongFangxin Liu

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