Apatite-mediated actin dynamics in resorbing osteoclasts

Molecular Biology of the Cell
Frédéric SaltelPierre Jurdic

Abstract

The actin cytoskeleton is essential for osteoclasts main function, bone resorption. Two different organizations of actin have been described in osteoclasts, the podosomes belt corresponding to numerous F-actin columns arranged at the cell periphery, and the sealing zone defined as a unique large band of actin. To compare the role of these two different actin organizations, we imaged osteoclasts on various substrata: glass, dentin, and apatite. Using primary osteoclasts expressing GFP-actin, we found that podosome belts and sealing zones, both very dynamic actin structures, were present in mature osteoclasts; podosome belts were observed only in spread osteoclasts adhering onto glass, whereas sealing zone were seen in apico-basal polarized osteoclasts adherent on mineralized matrix. Dynamic observations of several resorption cycles of osteoclasts seeded on apatite revealed that 1) podosomes do not fuse together to form the sealing zone; 2) osteoclasts alternate successive stationary polarized resorption phases with a sealing zone and migration, nonresorption phases without any specific actin structure; and 3) apatite itself promotes sealing zone formation though c-src and Rho signaling. Finally, our work suggests that apatite-me...Continue Reading

References

Aug 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·P T Lakkakorpi, H K Väänänen
Jun 29, 1988·Journal of Chromatography·F W Putnam, N Takahashi
Feb 1, 1996·Microscopy Research and Technique·P T Lakkakorpi, H K Väänänen
Dec 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·I NakamuraT Suda
Jun 1, 1999·European Journal of Cell Biology·S RazzoukG Cournot
Feb 19, 2000·Journal of Biomedical Materials Research·T ShibutaniY Iwayama
Feb 23, 2000·The Journal of Clinical Investigation·K P McHughS L Teitelbaum
Apr 15, 2000·The Journal of Biological Chemistry·M A ChellaiahK A Hruska
Sep 17, 2002·Calcified Tissue International·I KritsB S Lee
Dec 13, 2002·Nature·Sandrine Etienne-Manneville, Alan Hall
Jan 17, 2003·Molecular Biology of the Cell·M A ChellaiahK A Hruska
May 6, 2003·The Journal of Biological Chemistry·Meenakshi A ChellaiahKeith A Hruska
Jul 3, 2003·Trends in Cell Biology·Stefan Linder, Martin Aepfelbacher
Jul 25, 2003·Microscopy Research and Technique·Mika MulariH Kalervo Väänänen
Dec 31, 2003·The Journal of Biological Chemistry·Gen He, Anne George
Jan 24, 2004·The Journal of Biological Chemistry·Tsuyoshi MiyazakiRoland Baron

❮ Previous
Next ❯

Citations

Dec 19, 2007·Cell and Tissue Research·Toshitaka AkisakaKeiko Takama
Jul 31, 2007·Odontology·Naoyuki TakahashiHidehiro Ozawa
Jan 27, 2009·Cancer Metastasis Reviews·Mahmut Yilmaz, Gerhard Christofori
Jan 21, 2009·Cancer Metastasis Reviews·Roberto BuccioneInmaculada Ayala
Jul 5, 2008·Clinical & Experimental Metastasis·Katarina Wolf, Peter Friedl
Sep 16, 2006·Journal of Molecular Histology·Vanessa NicolinPaola Narducci
May 14, 2011·Journal of Materials Science. Materials in Medicine·J HaoS Kasugai
Dec 23, 2011·Journal of Materials Science. Materials in Medicine·Zahi BadranAssem Soueidan
May 13, 2006·Journal of Bioenergetics and Biomembranes·L Shannon HollidayJian Zuo
Sep 5, 2006·Reviews in Endocrine & Metabolic Disorders·Angela Bruzzaniti, Roland Baron
Jun 12, 2012·Applied Biochemistry and Biotechnology·Asha Anand, Pramod Kumar Srivastava
Jan 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Tobias HeckelBernard Hoflack
Jan 19, 2010·The Journal of Biological Chemistry·Brooke K McMichaelBeth S Lee
Jul 24, 2010·The Journal of Biological Chemistry·Tao MaMeenakshi A Chellaiah
Jul 13, 2006·The Journal of Biological Chemistry·John C EdwardsPaul H Schlesinger
Nov 26, 2008·The Journal of Biological Chemistry·Susan R WilsonDieter Brömme
Apr 14, 2006·Molecular Biology of the Cell·Shandiz TehraniJohn A Cooper
Sep 28, 2007·Molecular Biology of the Cell·Anne ChabadelFrédéric Saltel
Feb 26, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Firas M KaraBruce N Cronstein
Nov 18, 2011·Journal of the Royal Society, Interface·Dafna GeblingerBenjamin Geiger
Jun 1, 2005·Molecular and Cellular Biology·Nathan J PavlosMing H Zheng
Apr 9, 2010·Journal of Cell Science·Dafna GeblingerBenjamin Geiger
Aug 20, 2010·Journal of Cell Science·Wei ZouSteven L Teitelbaum
Feb 14, 2012·Journal of Cell Science·Chen LuxenburgBenjamin Geiger
Sep 24, 2010·PloS One·Karen FullerTim J Chambers
Nov 3, 2011·PloS One·Romain DacquinIrma Machuca-Gayet
Feb 4, 2012·PloS One·Nathalie SaulnierMurielle Gaudry
Oct 11, 2012·PloS One·Anne BlangyGeraldine Pawlak
Apr 5, 2013·PloS One·Kinga A SzewczykTim J Chambers
Sep 16, 2010·Journal of Molecular Endocrinology·Edith BonnelyePierre Jurdic
Aug 8, 2014·PloS One·Sujeeve JeganathanRene E Harrison
Nov 29, 2013·Molecular Biology of the Cell·Dan GeorgessIrma Machuca-Gayet
Jul 9, 2011·Molecular Biology of the Cell·Shiqiong HuJean-Christophe Géminard
Apr 1, 2010·European Journal of Histochemistry : EJH·P NarducciV Nicolin
Apr 20, 2013·European Journal of Cell Biology·Heiani TouaitahuataGeraldine Pawlak
Jun 8, 2012·European Journal of Cell Biology·Lucie BrissonSébastien Roger

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

European Journal of Cell Biology
Pierre JurdicOlivier Destaing
Trends in Cell Biology
Stefan Linder, Martin Aepfelbacher
Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
P T Lakkakorpi, H K Väänänen
© 2021 Meta ULC. All rights reserved