APC2 controls dendrite development by promoting microtubule dynamics

Nature Communications
Olga I KahnCasper C Hoogenraad

Abstract

Mixed polarity microtubule organization is the signature characteristic of vertebrate dendrites. Oppositely oriented microtubules form the basis for selective cargo trafficking in neurons, however the mechanisms that establish and maintain this organization are unclear. Here, we show that APC2, the brain-specific homolog of tumor-suppressor protein adenomatous polyposis coli (APC), promotes dynamics of minus-end-out microtubules in dendrites. We found that APC2 localizes as distinct clusters along microtubule bundles in dendrites and that this localization is driven by LC8-binding and two separate microtubule-interacting domains. Depletion of APC2 reduces the plus end dynamics of minus-end-out oriented microtubules, increases microtubule sliding, and causes defects in dendritic morphology. We propose a model in which APC2 regulates dendrite development by promoting dynamics of minus-end-out microtubules.

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Citations

Mar 21, 2020·Journal of Neurochemistry·Aleksandra TempesJacek Jaworski
Sep 6, 2019·EMBO Reports·Dieudonnée van de WilligeAnna Akhmanova
Mar 26, 2021·Frontiers in Molecular Neuroscience·Alberto Perez-AlvarezThomas G Oertner
Oct 3, 2020·Current Biology : CB·Maria Angeles JuanesBruce L Goode
Apr 5, 2021·Neuroscience Letters·Shrobona GuhaPeter W Baas

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Datasets Mentioned

BETA
GM130

Methods Mentioned

BETA
GTPases
acetylation
pull-downs
pull-down
pull down
FCS
PCR
transfection
chip
fluorescence recovery after photobleaching

Key Resources (RRID) Mentioned

AB_2040524
AB_10638135
AB_477256
AB_2138153
AB_303395
AB_2571743
AB_2120990
AB_2313990
AB_397554
AB_796193

Software Mentioned

ImageJ Fiji
Excel
KymoReslice
ImageJ
MetaMorph
Coloc
LAS X controlling
Split
Correlescence
SPSS

Related Concepts

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Adenomatous Polyposis Coli

Adenomatous polyposis coli is a protein encoded by the APC gene and acts as a tumor suppressor. Discover the latest research on adenomatous polyposis coli here.