Abstract
Methamphetamine (METH) is a potent psychomotor stimulant that has a high potential for abuse in humans. In addition, it is neurotoxic, especially in dopaminergic neurons. Long-lasting exposure to METH causes psychosis and increases the risk of Parkinson's disease. Apelin-13 is a novel endogenous ligand which studies have shown that may have a neuroprotective effect. Therefore, we hypothesized that Apelin-13 might adequately prevent METH-induced neurotoxicity via the inhibition of apoptotic, autophagy, and ROS responses. In this study, PC12 cells were exposed to both METH (0.5, 1, 2, 3, 4, 6 mmol/L) and Apelin-13 (0.5, 1.0, 2.0, 4.0, 8.0 μmol/L) in vitro for 24 h to measure determined dose, and then downstream pathways were measured to investigate apoptosis, autophagy, and ROS responses. The results have indicated that Apelin-13 decreased the apoptotic response post-METH exposure in PC12 cells by increasing cell viability, reducing apoptotic rates. In addition, the study has revealed Apelin-13 decreased gene expression of Beclin-1 by Real-Time PCR and LC3-II by western blotting in METH-induced PC12 cells, which demonstrated autophagy is reduced. In addition, this study has shown that Apelin-13 reduces intracellular ROS of METH-i...Continue Reading
References
Oct 28, 1998·Experimental Gerontology·R Sgonc, J Gruber
Nov 21, 2000·Annals of the New York Academy of Sciences·J W LauA Stadlin
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 23, 2004·Annals of the New York Academy of Sciences·M T OliveiraC R Oliveira
May 26, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Danièle DaviaudIsabelle Castan-Laurell
Jul 1, 2006·The AAPS Journal·Evan L RiddleGlen R Hanson
Aug 19, 2007·Basic Research in Cardiology·James C SimpkinChristopher C T Smith
Sep 5, 2007·Journal of Neurochemistry·Lauren A O'DonnellDennis L Kolson
Oct 6, 2007·Cell Death and Differentiation·Y ChenS B Gibson
Nov 7, 2007·European Journal of Endocrinology·Sajad AhmadizadMohammad Reza Hamedinia
Dec 23, 2008·Transplantation Proceedings·J MalyszkoM Mysliwiec
Mar 24, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Nicholas J LeeperThomas Quertermous
Feb 16, 2010·Experimental Cell Research·Xiang Jun ZengLing Wei
Jan 29, 2011·Primary Care Companion to the Journal of Clinical Psychiatry·Seyed Vahid Shariat, Adele Elahi
May 14, 2011·Public Health Reports·Natalia MelnikovaMartha Stanbury
Sep 3, 2011·PloS One·Atsushi KasaiSadaaki Maeda
May 29, 2012·Journal of Molecular Neuroscience : MN·Mehdi KhaksariZahra Madjd
May 31, 2012·Journal of Neuroinflammation·Vanessa Coelho-SantosAna Paula Silva
Jul 24, 2012·Peptides·Baohua ChengQing Xin
Oct 9, 2012·Toxicology Letters·Yan LiXiaobo Cen
Sep 17, 2013·Clinical Cardiology·Sekon WonNisha I Parikh
Mar 8, 2014·Cell Death & Disease·J MaS Roy
Mar 29, 2014·Nutrients·Marcelo P BarrosEduardo F Bondan
Oct 4, 2014·Peptides·Qing XinBo Bai
Nov 5, 2014·Neurochemical Research·Hai-Jun BaoDing-Kun Dai
Dec 3, 2014·Peptides·Yanjie YangQiang Chen
Jan 16, 2015·Neurotoxicity Research·Rujiraporn PitaksaleePimtip Sanvarinda
Apr 11, 2015·Behavioural Neurology·Shaobin YuXuhui Di
May 23, 2015·Frontiers in Neuroscience·Patric Turowski, Bridget-Ann Kenny
Oct 11, 2015·Neurochemical Research·Shou-Hong ZhouBi Hu
Jan 7, 2016·Molecular Neurobiology·Ya-Ni HuangJia-Yi Wang
Feb 14, 2016·Molecular Neurobiology·Bing LiHuijun Wang
Mar 11, 2016·Neurochemical Research·Yunjun ZouShaowen Tian
Apr 2, 2016·International Journal of Molecular Medicine·Qun ZhouLinxi Chen
Jul 16, 2016·Addiction Biology·Eun Young JangScott C Steffensen
Oct 25, 2016·Chemico-biological Interactions·Ali RoohbakhshGholamreza Karimi
Feb 19, 2017·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Maryam AkhgariAfshar Etemadi-Aleagha
Mar 1, 2017·Archives of Pharmacal Research·Eun-Joo ShinHyoung-Chun Kim
Mar 10, 2017·Neuroscience Letters·Xiang TianChaoying Li
May 31, 2017·Free Radical Research·Pu ZhangLian-Qun Cui
Jul 12, 2017·Frontiers in Molecular Neuroscience·Xiang XuWei-Bing Xie
Sep 28, 2018·Neurochemical Research·Monire ShafahiMehdi Khaksari
Oct 21, 2018·Nutrition Research·Qi XiongChaoying Li
Nov 21, 2018·Journal of Molecular Neuroscience : MN·Fateme GhanbariAbdolhossein Shiravi
Citations
Feb 23, 2020·Brain and Behavior·Yunle MengPingming Qiu
Apr 17, 2020·Frontiers in Aging Neuroscience·Yuki IshimaruSadaaki Maeda
May 10, 2020·Archives of Toxicology·Jorge SoaresJoão Paulo Capela
Nov 12, 2020·International Journal of Molecular Sciences·Peng ChenJunxia Xie
Nov 6, 2020·Phytochemistry·Qian HeYue-Hu Wang
Mar 1, 2021·Neuropeptides·Jia-Xiu ZhouShao-Wen Tian
Mar 20, 2021·Frontiers in Cell and Developmental Biology·Hao ZhangYuelin Zhang
Apr 4, 2021·International Journal of Molecular Sciences·Ewa MlyczyńskaAgnieszka Rak
Apr 6, 2021·Neural Regeneration Research·Ying WangLei Chen