Apelin-13 Protects PC12 Cells Against Methamphetamine-Induced Oxidative Stress, Autophagy and Apoptosis

Neurochemical Research
Kobra ForoughiAsghar Shayannia

Abstract

Methamphetamine (METH) is a potent psychomotor stimulant that has a high potential for abuse in humans. In addition, it is neurotoxic, especially in dopaminergic neurons. Long-lasting exposure to METH causes psychosis and increases the risk of Parkinson's disease. Apelin-13 is a novel endogenous ligand which studies have shown that may have a neuroprotective effect. Therefore, we hypothesized that Apelin-13 might adequately prevent METH-induced neurotoxicity via the inhibition of apoptotic, autophagy, and ROS responses. In this study, PC12 cells were exposed to both METH (0.5, 1, 2, 3, 4, 6 mmol/L) and Apelin-13 (0.5, 1.0, 2.0, 4.0, 8.0 μmol/L) in vitro for 24 h to measure determined dose, and then downstream pathways were measured to investigate apoptosis, autophagy, and ROS responses. The results have indicated that Apelin-13 decreased the apoptotic response post-METH exposure in PC12 cells by increasing cell viability, reducing apoptotic rates. In addition, the study has revealed Apelin-13 decreased gene expression of Beclin-1 by Real-Time PCR and LC3-II by western blotting in METH-induced PC12 cells, which demonstrated autophagy is reduced. In addition, this study has shown that Apelin-13 reduces intracellular ROS of METH-i...Continue Reading

References

Oct 28, 1998·Experimental Gerontology·R Sgonc, J Gruber
Nov 21, 2000·Annals of the New York Academy of Sciences·J W LauA Stadlin
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 23, 2004·Annals of the New York Academy of Sciences·M T OliveiraC R Oliveira
May 26, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Danièle DaviaudIsabelle Castan-Laurell
Jul 1, 2006·The AAPS Journal·Evan L RiddleGlen R Hanson
Aug 19, 2007·Basic Research in Cardiology·James C SimpkinChristopher C T Smith
Sep 5, 2007·Journal of Neurochemistry·Lauren A O'DonnellDennis L Kolson
Nov 7, 2007·European Journal of Endocrinology·Sajad AhmadizadMohammad Reza Hamedinia
Mar 24, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Nicholas J LeeperThomas Quertermous
Jan 29, 2011·Primary Care Companion to the Journal of Clinical Psychiatry·Seyed Vahid Shariat, Adele Elahi
Jul 24, 2012·Peptides·Baohua ChengQing Xin
Sep 17, 2013·Clinical Cardiology·Sekon WonNisha I Parikh
Nov 5, 2014·Neurochemical Research·Hai-Jun BaoDing-Kun Dai
Dec 3, 2014·Peptides·Yanjie YangQiang Chen
Jan 16, 2015·Neurotoxicity Research·Rujiraporn PitaksaleePimtip Sanvarinda
May 23, 2015·Frontiers in Neuroscience·Patric Turowski, Bridget-Ann Kenny
Apr 2, 2016·International Journal of Molecular Medicine·Qun ZhouLinxi Chen
Oct 25, 2016·Chemico-biological Interactions·Ali RoohbakhshGholamreza Karimi
Feb 19, 2017·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Maryam AkhgariAfshar Etemadi-Aleagha
Mar 1, 2017·Archives of Pharmacal Research·Eun-Joo ShinHyoung-Chun Kim
Mar 10, 2017·Neuroscience Letters·Xiang TianChaoying Li
Nov 21, 2018·Journal of Molecular Neuroscience : MN·Fateme GhanbariAbdolhossein Shiravi

❮ Previous
Next ❯

Citations

Apr 17, 2020·Frontiers in Aging Neuroscience·Yuki IshimaruSadaaki Maeda
Mar 1, 2021·Neuropeptides·Jia-Xiu ZhouShao-Wen Tian

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
electrophoresis
flow cytometry
PCR

Software Mentioned

Image J
GraphPad
GraphPad Prism

Related Concepts

Related Feeds

Autophagy & Disease

Autophagy is an important cellular process for normal physiology and both elevated and decreased levels of autophagy are associated with disease. Here is the latest research.

Parkinson's Disease & Autophagy (MDS)

Autophagy leads to degradation of damaged proteins and organelles by the lysosome. Impaired autophagy has been implicated in several diseases. Here is the role of autophagy in Parkinson’s disease.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Parkinson's Disease & Autophagy

Autophagy leads to degradation of damaged proteins and organelles by the lysosome. Impaired autophagy has been implicated in several diseases. Here is the role of autophagy in Parkinson’s disease.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis