Apelin Affects the Progression of Osteoarthritis by Regulating VEGF-Dependent Angiogenesis and miR-150-5p Expression in Human Synovial Fibroblasts.

Cells
Yu-Han WangChih-Hsin Tang

Abstract

Synovium-induced angiogenesis is central to osteoarthritis (OA) pathogenesis and thus a promising therapeutic target. The adipokine apelin (APLN) is involved in both OA pathogenesis and angiogenesis. We examined the role of APLN in synovium-induced angiogenesis by investigating the crosstalk between APLN and vascular endothelial growth factor (VEGF) expression in human OA synovial fibroblasts (OASFs). We found higher levels of APLN and VEGF expression in OA samples compared with normal samples. APLN-induced stimulation of VEGF expression and VEGF-dependent angiogenesis in OASFs was mitigated by FAK/Src/Akt signaling. APLN also inhibited levels of microRNA-150-5p (miR-150-5p), which represses VEGF production and angiogenesis. Analyses of an OA animal model showed that shAPLN transfection of OASFs rescued pathologic changes in OA cartilage and histology. Here, we found APLN enhances VEGF expression and angiogenesis via FAK/Src/Akt cascade and via downstream suppression of miR-150-5p expression. These findings help to clarify the pathogenesis of adipokine-induced angiogenesis in OA synovium.

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Citations

Aug 14, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Wei-Cheng ChenChih-Hsin Tang
Nov 14, 2020·Current Opinion in Rheumatology·Natalia Zapata-LinaresXavier Houard

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Datasets Mentioned

BETA
GSE46750

Methods Mentioned

BETA
ELISA
PCR
electrophoresis
transfection
Assay

Software Mentioned

miRMap
RNAhybrid
GraphPad
Skyscan
miRWalk
StepOnePlus
TreeView
TargetScan
MacBiophotonics ImageJ
GraphPad Prism

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