Apelin decreases the SR Ca2+ content but enhances the amplitude of [Ca2+]i transient and contractions during twitches in isolated rat cardiac myocytes

American Journal of Physiology. Heart and Circulatory Physiology
Chen WangHai-Chang Wang

Abstract

Apelin has been reported to have a positive inotropic action in the isolated rat heart. However, the effect of apelin on sarcoplasmic reticulum (SR) Ca2+ content and its influence on intracellular Ca2+ transient during excitation-contraction coupling remains poorly understood. In the present study, we determined the effect of apelin on Ca2+ transient and contractions in isolated rat cardiomyocytes. When compared with control, treatment with apelin caused a 55.7 +/- 13.9% increase in sarcomere fraction shortening and a 43.6 +/- 4.56% increase in amplitude of electrical-stimulated intracellular Ca2+ concentration (E[Ca2+]i) transients (n = 14, P < 0.05). But SR Ca2+ content measured by caffeine-induced [Ca2+]i (C[Ca2+]i) transient was decreased 8.41 +/- 0.92% in response to apelin (n = 14, P < 0.05). Na+/Ca2+ exchanger (NCX) function was increased since half-decay time of C[Ca2+]i was decreased 16.22 +/- 1.36% in response to apelin. Sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) activity was also increased by apelin. These responses can be partially or completely blocked by chelerythrine chloride, a PKC inhibitor. In addition, to confirm our data, we used indo-1 as another Ca2+ indicator and rapid cooling as another way to meas...Continue Reading

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Citations

Jun 2, 2012·Cardiology Research and Practice·Seyyed M R Kazemi-BajestaniGavin Y Oudit
Oct 16, 2015·Journal of Receptor and Signal Transduction Research·Sinan CanpolatAhmet Ayar
Oct 19, 2010·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Cao ZouXiangjun Yang
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Aug 15, 2021·Nature Reviews. Nephrology·Fiona A ChapmanNeeraj Dhaun

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