Aphidicolin-induced FRA3B breakpoints cluster in two distinct regions

Genomics
L WangD I Smith

Abstract

The common fragile site at chromosomal band 3p14.2 (FRA3B) is the most sensitive single site in the human genome to induced chromosomal lesions. This fragile site may predispose chromosome 3p to breakage that is commonly observed in lung, renal, and many other cancers. We previously used aphidicolin induction of FRA3B expression in a chromosome 3-only somatic cell hybrid to generate a series of hybrids with breakpoints in the 3p14.2 region. These breakpoints were localized to two distinct clusters, separated by 200 kb, that lie on either side of a region of frequent breakage within FRA3B as observed by FISH analysis. Seven proximal aphidicolin-induced breakpoints were localized at or near the end of a THE element. The THE-1 element is flanked by LINE and Alu repetitive elements. The eight distal aphidicolin-induced breakpoints clustered in a region capable of forming multiple hairpin-like structures. Thus repetitive elements and hairpin-like structures may be responsible for chromosome fragility in this region.

References

Sep 13, 1979·The New England Journal of Medicine·A J CohenR S Brown
May 1, 1985·Annals of Human Genetics·G R Sutherland
Dec 7, 1984·Science·J J Yunis, A L Soreng
Apr 1, 1984·Cancer Genetics and Cytogenetics·N Wang, K L Perkins
Oct 17, 1995·Biochimica Et Biophysica Acta·S ChongA A Piper

❮ Previous
Next ❯

Citations

Jan 2, 2008·Cell Research·Irina V Kovtun, Cynthia T McMurray
Apr 1, 1998·Journal of the National Cancer Institute·G A OttersonR S Yeung
Jul 3, 2004·Genome Research·David J Mulvihill, Yuh-Hwa Wang
Feb 3, 1999·Annual Review of Genetics·K HuebnerC M Croce
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·K MimoriC M Croce
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·H InoueC M Croce
Apr 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·T ShiraishiC M Croce
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·D MishmarB Kerem
Dec 15, 2007·Biochimica Et Biophysica Acta·T Lukusa, J P Fryns
Oct 19, 2005·Cancer Letters·Yuh-Hwa Wang
Oct 21, 2005·Cancer Letters·Michal SchwartzBatsheva Kerem
Oct 5, 2014·Experimental Cell Research·Abdelghani MazouziJoanna I Loizou
Jan 16, 2003·Cell·Anne M CasperThomas W Glover
Jun 22, 2016·Molecular Cytogenetics·Mónica Villalba-CamposMilena Rondón-Lagos
Jul 17, 2001·Journal of Cellular Biochemistry. Supplement·E Y SvetlovaM Debatisse
Dec 17, 2002·Oncogene·Nicole A BeckerDavid I Smith
Jul 16, 2004·Molecular and Cellular Biology·Martin F ArltThomas W Glover
May 16, 2018·Frontiers in Molecular Neuroscience·Gulfem D GulerJeannine Gerhardt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.