Apiaceous vegetable consumption decreases PhIP-induced DNA adducts and increases methylated PhIP metabolites in the urine metabolome in rats

The Journal of Nutrition
Jae Kyeom KimSabrina P Trudo

Abstract

Heterocyclic aromatic amines, such as 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), are carcinogenic compounds produced during heating of protein-containing foods. Apiaceous vegetables inhibit PhIP-activating enzymes, whereas cruciferous vegetables induce both PhIP-activating and -detoxifying enzymes. We investigated the effects of these vegetables, either alone or combined, on PhIP metabolism and colonic DNA adduct formation in rats. Male Wistar rats were fed cruciferous vegetables (21%, wt:wt), apiaceous vegetables (21%, wt:wt), or a combination of both vegetables (10.5% wt:wt of each). Negative and positive control groups were fed an AIN-93G diet. After 6 d, all groups received an intraperitoneal injection of PhIP (10 mg · kg body weight(-1)) except for the negative control group, which received only vehicle. Urine was collected for 24 h after the injection for LC-tandem mass spectrometry metabolomic analyses. On day 7, rats were killed and tissues processed. Compared with the positive control, cruciferous vegetables increased the activity of hepatic PhIP-activating enzymes [39.5% and 45.1% for cytochrome P450 (CYP) 1A1 (P = 0.0006) and CYP1A2 (P < 0.0001), respectively] and of uridine 5'-diphospho-glucuronosyltran...Continue Reading

References

Jan 1, 1992·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·H M WortelboerH E Falke
Sep 1, 1989·Mutation Research·J D TuckerJ S Felton
Apr 1, 1995·Pharmacogenetics·A CatteauP Laurent
Jan 20, 1998·Drug and Chemical Toxicology·M M Doherty, K S Pang
Mar 29, 2001·Journal of Pharmaceutical and Biomedical Analysis·N HaniokaM Ando
Apr 16, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·U Kristina Walle, Thomas Walle
Apr 18, 2002·Journal of Agricultural and Food Chemistry·Edwin OstertagDieter Schrenk
Jun 18, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·E MuckelH R Glatt
Sep 28, 2002·Mutation Research·Robert J TureskySophie Langouët
Oct 9, 2002·The Journal of Nutrition·Robert Rucker, David Storms
Mar 5, 2003·American Journal of Epidemiology·L M ButlerR S Sandler
Mar 12, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·S FukushimaF Furukawa
Apr 10, 2004·Cancer Science·Takashi SugimuraMinako Nagao
Apr 29, 2004·The American Journal of Clinical Nutrition·Claudine ManachLiliana Jiménez
Aug 3, 2004·Nature Reviews. Cancer·Miriam C Poirier
Sep 8, 2004·Biochemical and Biophysical Research Communications·Smarajit MaitiGuangping Chen
Jan 27, 2005·Biochemical Pharmacology·Annette BaumgartDieter Schrenk
Sep 6, 2005·Cancer Research·Rashmi SinhaRichard B Hayes
Jun 10, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Sabrina PetersonDavid L Eaton
Feb 22, 2008·The Journal of Nutrition·Andrea Y Arikawa, Daniel D Gallaher
Jun 23, 2009·Journal of Pharmaceutical Sciences·Ana I AlvarezGracia Merino
Oct 22, 2009·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Sabrina PetersonJohanna W Lampe
Jul 14, 2010·Molecular Nutrition & Food Research·Urvi Telang, Marilyn E Morris
Jan 27, 2011·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Shogo J Miyagi, Abby C Collier

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Citations

Feb 1, 2017·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Mengyao ZhaoFang Chen

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