Apical accumulation of MARCKS in neural plate cells during neurulation in the chick embryo

BMC Developmental Biology
Flavio R Zolessi, Cristina Arruti

Abstract

The neural tube is formed by morphogenetic movements largely dependent on cytoskeletal dynamics. Actin and many of its associated proteins have been proposed as important mediators of neurulation. For instance, mice deficient in MARCKS, an actin cross-linking membrane-associated protein that is regulated by PKC and other kinases, present severe developmental defects, including failure of cranial neural tube closure. To determine the distribution of MARCKS, and its possible relationships with actin during neurulation, chick embryos were transversely sectioned and double labeled with an anti-MARCKS polyclonal antibody and phalloidin. In the neural plate, MARCKS was found ubiquitously distributed at the periphery of the cells, being conspicuously accumulated in the apical cell region, in close proximity to the apical actin meshwork. This asymmetric distribution was particularly noticeable during the bending process. After the closure of the neural tube, the apically accumulated MARCKS disappeared, and this cell region became analogous to the other peripheral cell zones in its MARCKS content. Actin did not display analogous variations, remaining highly concentrated at the cell subapical territory. The transient apical accumulation ...Continue Reading

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Citations

Jan 20, 2016·Cytoskeleton·Sérgio Carvalho Leite, Mónica Mendes Sousa
Feb 27, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Hiroaki Okae, Yoichiro Iwakura
Apr 25, 2012·Development, Growth & Differentiation·Makoto SuzukiNaoto Ueno
Mar 18, 2016·Biomechanics and Modeling in Mechanobiology·Alina OlteanLarry A Taber
Aug 25, 2016·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Daniel Prieto, Flavio R Zolessi
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Mar 29, 2014·Histochemistry and Cell Biology·Daniel PrietoFlavio R Zolessi
Jul 17, 2015·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Claudio ArayaMiguel Miranda
Sep 24, 2019·PLoS Genetics·Ding YeYonghua Sun
Mar 9, 2018·Frontiers in Neuroscience·Florence A Giger, Corinne Houart
Apr 1, 2018·Genesis : the Journal of Genetics and Development·Gonzalo AparicioFlavio R Zolessi
Oct 25, 2001·Brain Research. Developmental Brain Research·F R Zolessi, C Arruti
Sep 16, 2009·Developmental Biology·Jacob M SawyerBob Goldstein

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Methods Mentioned

BETA
fluorescence microscopy
transgenic

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