Apical constriction is driven by a pulsatile apical myosin network in delaminating Drosophila neuroblasts

Development
Yanru AnYan Yan

Abstract

Cell delamination is a conserved morphogenetic process important for the generation of cell diversity and maintenance of tissue homeostasis. Here, we used Drosophila embryonic neuroblasts as a model to study the apical constriction process during cell delamination. We observe dynamic myosin signals both around the cell adherens junctions and underneath the cell apical surface in the neuroectoderm. On the cell apical cortex, the nonjunctional myosin forms flows and pulses, which are termed medial myosin pulses. Quantitative differences in medial myosin pulse intensity and frequency are crucial to distinguish delaminating neuroblasts from their neighbors. Inhibition of medial myosin pulses blocks delamination. The fate of a neuroblast is set apart from that of its neighbors by Notch signaling-mediated lateral inhibition. When we inhibit Notch signaling activity in the embryo, we observe that small clusters of cells undergo apical constriction and display an abnormal apical myosin pattern. Together, these results demonstrate that a contractile actomyosin network across the apical cell surface is organized to drive apical constriction in delaminating neuroblasts.

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Citations

Mar 25, 2018·Nature Communications·Xiang QinXiaobo Wang
Dec 1, 2017·Development·Natalie C Heer, Adam C Martin
May 10, 2019·Cell Cycle·Tara M Finegan, Dan T Bergstralh
Mar 10, 2020·F1000Research·Ann Sutherland, Alyssa Lesko
Sep 4, 2020·Development·Hui Miao, J Todd Blankenship
Jan 6, 2021·Current Biology : CB·Youmna AtiehGeorge T Eisenhoffer
Feb 21, 2021·Nature Communications·Gabriel L GaleaAndrew J Copp
Feb 26, 2020·Current Biology : CB·Alexis Villars, Romain Levayer
Aug 31, 2021·Annual Review of Genetics·Nikhil Mishra, Carl-Philipp Heisenberg

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