Apical endocytosis in outer hair cells of the mammalian cochlea

The European Journal of Neuroscience
C B GriesingerJonathan F Ashmore

Abstract

Outer hair cells (OHCs), the sensory-motor cells of the mammalian cochlea, contain an endocytic tubulovesicular compartment below their apical stereocilia. We have used two-photon imaging of FM1-43 in the intact epithelium to show that these cells take up membrane in a Ca(2+)-dependent manner from a distinct apical site. The uptake rate was 0.8 microm(2)/s and internalized membrane was trafficked rapidly to a compartment along the lateral wall and distinct intracellular compartments. Double labelling with FM1-43 and DiOC(6), an endoplasmic reticulum (ER) marker, showed that these compartments are part of the tubulovesicular endoplasmic reticulum of OHCs. Labelling with a lysosomal marker showed that OHC lysosomes are restricted to the apex. Using the protein marker wheat germ agglutinin (WGA-FITC) we demonstrate that apical protein internalization and trafficking is about eight times slower than membrane internalization. Using double labelling with FM1-43 and WGA-FITC, we show that membrane and protein internalization are apically colocalized but that patterns of protein and membrane traffic differ. Protein was targeted only to the most apical third of the lateral wall. In control conditions, OHCs displayed only weak WGA-FITC s...Continue Reading

References

Jan 1, 1992·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·P K PlinkertH P Zenner
Mar 1, 1990·Brain Research. Developmental Brain Research·J J PrietoJ A Merchan
Jan 1, 1987·Hearing Research·P A Leake, R L Snyder
Jul 15, 1988·Hearing Research·P Gil-Loyzaga, W E Brownell
Jun 1, 1986·Journal of Neurocytology·J H Siegel, W E Brownell
Aug 1, 1994·Current Opinion in Cell Biology·K Matter, I Mellman
Nov 1, 1993·Hearing Research·P A Pollice, W E Brownell
Jul 1, 1996·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S Nishikawa, F Sasaki
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·A W HenkelW J Betz
Jul 25, 1996·Biochimica Et Biophysica Acta·Z MamdouhC Le Grimellec
Jun 1, 1996·Current Opinion in Neurobiology·W J BetzC B Smith
Jan 1, 1996·Annual Review of Cell and Developmental Biology·I Mellman
Jul 1, 1997·Physiological Reviews·S MukherjeeF R Maxfield
Dec 31, 1997·Cell·H RiezmanM Marsh
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·S S VogelN A Lambert
Jun 24, 1999·The Journal of Comparative Neurology·S S SpicerB A Schulte
Jun 10, 2000·Molecular Biology of the Cell·S M LeungG Apodaca
Jun 30, 2000·Current Opinion in Cell Biology·K E MostovY Altschuler
Dec 18, 2001·Hearing Research·J MeyerA W Gummer
Apr 6, 2002·Histochemistry and Cell Biology·Monika VetterleinMargit Pavelka

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Citations

Aug 19, 2007·Journal of the Association for Research in Otolaryngology : JARO·Anping XiaJohn S Oghalai
Jun 12, 2009·Journal of the Association for Research in Otolaryngology : JARO·Louise E Organ, Robert M Raphael
Dec 13, 2012·Molecular Therapy. Nucleic Acids·Suvarna Dash-WaghMats Ulfendahl
Jan 4, 2013·PloS One·Kateri J Spinelli, Peter G Gillespie
Nov 23, 2013·Journal of the Association for Research in Otolaryngology : JARO·Benjamin R Thiede, Jeffrey T Corwin
May 28, 2014·The Journal of Cell Biology·Natalia H ReveloSilvio O Rizzoli
Mar 11, 2009·Neuroscience·M A CrumlingR K Duncan
Jul 5, 2006·The European Journal of Neuroscience·Toshihiko KanekoAnthony W Gummer
Oct 19, 2006·Hearing Research·Jean VautrinClaude J Dechesne
Feb 6, 2015·Human Molecular Genetics·Misato YoshikawaJin-ichi Inokuchi
Oct 27, 2007·Free Radical Research·Xiaorui ShiAlfred Nuttall
Apr 25, 2007·Molecular Membrane Biology·Xiaorui ShiAlfred L Nuttall
Feb 4, 2005·American Journal of Physiology. Cell Physiology·Xiaorui ShiAlfred L Nuttall
Jul 6, 2014·Physiological Reviews·Robert Fettiplace, Kyunghee X Kim
May 8, 2021·Frontiers in Molecular Neuroscience·Luis Colón-CruzMartine Behra
May 28, 2021·The European Journal of Neuroscience·Csaba HarasztosiAnthony W Gummer

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