Apical localization of actin patches and vacuolar dynamics in Ashbya gossypii depend on the WASP homolog Wal1p

Journal of Cell Science
Andrea Walther, Jürgen Wendland

Abstract

Analysis of the Ashbya gossypii Wiskott-Aldrich syndrome-like gene AgWAL1 indicates that it is required for the maintenance of polarized hyphal growth. Growth and organelle dynamics of the wild type and of wal1 and other mutant strains were monitored by in vivo (fluorescence) time-lapse microscopy. Loss of WAL1 led to slow growth and defects in polarized growth that produced swellings in subapical regions, whereas formation of hyphal tips and dichotomous tip branching occurred as in the wild-type. Few actin cables in Agwal1 cells were found to insert into the hyphal tip, but specific clustering of cortical actin patches was observed in subapical regions of hyphal tips instead of at the hyphal apex. Distribution and movement of vacuoles was observed in vivo using FM4-64. In the wild type and in the slowly growing mutant strains bem2 and cla4, which lack a Rho-GTPase-activating protein and a PAK kinase, respectively, early endosomes appeared in the hyphal tip, whereas very few early endosomes and small vacuoles were found in the wal1 mutant hyphal tips, thus linking the cortical patch defect of wal1 hyphae with the distribution of endosomes. Vivid movement of vacuoles seen in the wild type and in the bem2 mutant in subapical regi...Continue Reading

References

Feb 9, 1996·Cell·D G Drubin, W J Nelson
Jul 1, 1997·Current Biology : CB·J A Frazier, C M Field
Jan 19, 1999·Annual Review of Cell and Developmental Biology·A Schmidt, M N Hall
Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·D I Johnson
Mar 24, 1999·Trends in Cell Biology·N RameshR S Geha
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·L M MacheskyT D Pollard
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·D C WinterR Li
Feb 9, 2000·The Journal of Cell Biology·J TauntonC A Larabell
Apr 3, 2001·Applied and Environmental Microbiology·M K Watters, A J Griffiths
Nov 1, 2001·Fungal Genetics and Biology : FG & B·J Wendland
Dec 12, 2001·Nature Cell Biology·Marie EvangelistaAanthony Bretscher
Jan 17, 2002·Current Opinion in Cell Biology·Emmanuelle Caron
Feb 21, 2002·Molecular Biology of the Cell·Kathryn E Sharpless, Steven D Harris
Jul 23, 2002·Nature Cell Biology·Isabelle SagotDavid Pellman
Sep 12, 2002·Fungal Genetics and Biology : FG & B·Jürgen Wendland, Peter Philippsen
Nov 29, 2002·Eukaryotic Cell·U OberholzerM Whiteway
Nov 30, 2002·Current Opinion in Microbiology·Michelle Momany
Dec 13, 2002·Nature·Sandrine Etienne-Manneville, Alan Hall
Jan 9, 2003·Current Opinion in Cell Biology·Alissa M WeaverJohn A Cooper
Mar 21, 2003·Current Biology : CB·Fanny S ChangKendall J Blumer
May 31, 2003·Journal of Cell Science·Marie EvangelistaCharles Boone
Jun 20, 2003·Current Biology : CB·Avital A RodalDavid G Drubin
Nov 6, 2003·Fungal Genetics and Biology : FG & B·Andrea Walther, Jürgen Wendland
Nov 18, 2003·Annual Review of Genetics·Lois S Weisman
Apr 7, 2004·Journal of Molecular Biology·Matthias BuckMichael K Rosen

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Citations

May 5, 2007·Current Genetics·Alexander Dünkler, Jürgen Wendland
Apr 12, 2005·Nature Reviews. Microbiology·Jürgen Wendland, Andrea Walther
Oct 11, 2005·Eukaryotic Cell·Ronny MartinJürgen Wendland
Nov 26, 2008·Molecular and Cellular Biology·Andreas Kaufmann, Peter Philippsen
Mar 2, 2016·Bioscience, Biotechnology, and Biochemistry·Hiro-Omi HoshiHiroyuki Horiuchi
Dec 28, 2005·Mycological Research·Aleksandra Virag, Steven D Harris
Jul 22, 2005·European Journal of Cell Biology·Boris VoigtDiedrik Menzel
Jul 19, 2005·Current Opinion in Microbiology·Peter PhilippsenHans-Peter Schmitz
Oct 6, 2010·Current Opinion in Microbiology·Miguel Ángel Peñalva
May 20, 2014·Current Opinion in Microbiology·Gero Steinberg
Apr 13, 2010·Fungal Genetics and Biology : FG & B·Anke GrünlerJürgen Wendland
Jan 18, 2011·Microbiological Research·Sigyn JordeJürgen Wendland
Jun 1, 2016·Frontiers in Microbiology·Anna BergsNorio Takeshita
Aug 31, 2006·International Review of Cytology·Steven D Harris
Jun 13, 2015·PloS One·Erik VerschuerenBen Distel
Jun 19, 2013·Eukaryotic Cell·Romain GibeauxPeter Philippsen
Nov 4, 2011·Nature Reviews. Microbiology·Adokiye BerepikiNick D Read
Jul 9, 2013·Genetics·Lisa WasserstromJürgen Wendland
Nov 10, 2017·FEMS Microbiology Letters·Therese OscarssonJürgen Wendland
Sep 3, 2020·Journal of Fungi·Jürgen Wendland
Feb 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jorge A Leyva-RojasRüdiger Hampp
Jan 13, 2017·Molecular Biology of the Cell·Romain GibeauxFrançois Nédélec

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