PMID: 7541082Mar 1, 1995Paper

Apical membrane sodium and chloride entry during osmotic swelling of renal (A6) epithelial cells

The Journal of Membrane Biology
W E CroweN K Wills

Abstract

To assess the role of chloride in cell volume and sodium transport regulation, we measured cell height changes (CH), transepithelial chloride and sodium fluxes, and intracellular chloride content during challenge with hyposmotic solutions under open circuit (OC) conditions. CH maximally increased following hyposmotic challenge within approximately 5 minutes. The change in CH was smaller under short circuit (SC) conditions or following replacement of chloride in the mucosal solution by gluconate or cyclamate (Cl(-)-freem). When corrected for the osmotically inactive cell volume (30 +/- 2%), delta CH for controls (OC) were greater than predicted for an ideal osmometer. In contrast, delta CH for Cl(-)-freem or SC conditions were similar to that predicted for an ideal osmometer. Na+ and Cl- mucosa-to-serosa fluxes increased following hyposmotic challenge. Chloride fluxes increased maximally within 5 min, then decreased. In contrast, the Na+ flux increased slowly and reached a steady state after approximately 25 min. Under isosmotic conditions, exposure to Cl(-)-freem solutions led to decreases in the transepithelial conductance, Na+ flux, and CH. Chloride permeabilities in the apical and basolateral membranes were detected using th...Continue Reading

Citations

Nov 1, 1995·Pflügers Archiv : European journal of physiology·E BrochieroJ Ehrenfeld
Aug 24, 1999·The Journal of Physiology·U BanderaliJ Ehrenfeld
Mar 7, 2000·Toxicology and Applied Pharmacology·B Faurskov, H F Bjerregaard
Apr 29, 1998·Annual Review of Physiology·M CereijidoR G Contreras
Dec 6, 2002·Microscopy Research and Technique·Charlotte Andersson, Godfried M Roomans
Oct 23, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Max J LabJulia Gorelik
Mar 9, 2007·American Journal of Physiology. Renal Physiology·Akiyuki TarunoYoshinori Marunaka
Nov 14, 1997·The American Journal of Physiology·S LindenthalN K Wills
Feb 11, 1998·Physiological Reviews·F LangD Häussinger
Oct 1, 1998·The American Journal of Physiology·H AmlalM Soleimani
Mar 14, 2001·American Journal of Physiology. Cell Physiology·M B ButterworthW J Els
Sep 4, 2004·American Journal of Physiology. Renal Physiology·Danny JansWilly Van Driessche
Jun 13, 2002·American Journal of Physiology. Renal Physiology·David J RozanskyDavid Pearce
Aug 5, 2004·American Journal of Physiology. Renal Physiology·Naomi NiisatoYoshinori Marunaka
Jul 20, 2021·Journal of Cellular Physiology·Jun ZhangZhi-Ren Zhang

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