PMID: 9531552May 16, 1998Paper

Apical plasma membrane proteins and endolyn-78 travel through a subapical compartment in polarized WIF-B hepatocytes

The Journal of Cell Biology
Gudrun IhrkeAnn L Hubbard

Abstract

We studied basolateral-to-apical transcytosis of three classes of apical plasma membrane (PM) proteins in polarized hepatic WIF-B cells and then compared it to the endocytic trafficking of basolaterally recycling membrane proteins. We used antibodies to label the basolateral cohort of proteins at the surface of living cells and then followed their trafficking at 37 degreesC by indirect immunofluorescence. The apical PM proteins aminopeptidase N, 5'nucleotidase, and the polymeric IgA receptor were efficiently transcytosed. Delivery to the apical PM was confirmed by microinjection of secondary antibodies into the bile canalicular-like space and by EM studies. Before acquiring their apical steady-state distribution, the trafficked antibodies accumulated in a subapical compartment, which had a unique tubulovesicular appearance by EM. In contrast, antibodies to the receptors for asialoglycoproteins and mannose-6-phosphate or to the lysosomal membrane protein, lgp120, distributed to endosomes or lysosomes, respectively, without accumulating in the subapical area. However, the route taken by the endosomal/lysosomal protein endolyn-78 partially resembled the transcytotic pathway, since anti-endolyn-78 antibodies were found in a subapic...Continue Reading

References

Jan 1, 1992·Cold Spring Harbor Symposia on Quantitative Biology·K SimonsC Dotti
Dec 1, 1992·The Journal of Cell Biology·M J SchellA L Hubbard
Jan 1, 1992·Annual Review of Cell Biology·E Rodriguez-Boulan, S K Powell
Aug 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D Z Zhu, B U Pauli
Aug 1, 1991·The Journal of Membrane Biology·E SchaererJ P Kraehenbuhl
Oct 1, 1989·European Journal of Biochemistry·J QuintartP J Courtoy
Dec 1, 1989·The Journal of Cell Biology·M BomselK Simons
Mar 1, 1989·The Histochemical Journal·K T Tokuyasu
May 1, 1986·The Journal of Cell Biology·J Lippincott-Schwartz, D M Fambrough
Apr 1, 1985·The Journal of Cell Biology·J R BartlesA L Hubbard
Apr 1, 1985·The Journal of Cell Biology·A L HubbardL T Braiterman
Jun 1, 1985·The Journal of Cell Biology·V LewisI Mellman
Jan 1, 1985·Annual Review of Cell Biology·K Simons, S D Fuller
Aug 1, 1969·The Journal of Cell Biology·M A Venkatachalam, H D Fahimi
Mar 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·H G Coon, M C Weiss
Nov 1, 1972·The Journal of Cell Biology·A L Hubbard, Z A Cohn
Jul 15, 1984·The Biochemical Journal·E M BailyesJ P Luzio
Aug 1, 1995·Current Opinion in Cell Biology·J Gruenberg, F R Maxfield
Dec 1, 1993·The Journal of Cell Biology·G IhrkeA L Hubbard
Sep 29, 1995·The Journal of Biological Chemistry·L K VogelH Sjöström
Feb 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K E Mostov, M H Cardone

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Citations

Jun 27, 2000·Journal of Cellular Physiology·S C Van IJzendoornD Hoekstra
May 5, 2012·Purinergic Signalling·Herbert ZimmermannNorbert Sträter
Jun 12, 2003·Methods : a Companion to Methods in Enzymology·David Cohen, Anne Müsch
Mar 22, 2002·Molecular Biology of the Cell·Steven T TruschelGerard Apodaca
Oct 8, 1999·Molecular Biology of the Cell·S C van IJzendoorn, D Hoekstra
Jun 10, 2000·Molecular Biology of the Cell·S M LeungG Apodaca
Jul 9, 2004·Molecular Biology of the Cell·Sven C D Van IJzendoornDick Hoekstra
Apr 13, 2007·Molecular Biology of the Cell·Delphine ThéardSven C D van Ijzendoorn
Feb 22, 2008·Molecular Biology of the Cell·Lauren Henry, David R Sheff
Feb 5, 2002·Annual Review of Physiology·Helmut Kipp, Irwin M Arias
Apr 2, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·L BraitermanA L Hubbard
May 17, 2011·PloS One·Raquel Bello-MoralesJosé Antonio López-Guerrero
Apr 21, 2012·Journal of the American Society of Nephrology : JASN·Andrew C FryFiona E Karet Frankl
Oct 29, 2013·Annual Review of Pharmacology and Toxicology·Nathan D PfeiferKim L R Brouwer
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Lelita T BraitermanAnn L Hubbard
Apr 17, 2012·International Journal of Hepatology·Benita L McVickerCarol A Casey
Oct 20, 1999·The Journal of Cell Biology·N J QuintyneT A Schroer
Oct 2, 2002·FEBS Letters·Tounsia Aït Slimane, Dick Hoekstra
Sep 25, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·V BenderD Cassio
Oct 28, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Janet M LarkinVu Tinh
Oct 28, 2006·Alcoholism, Clinical and Experimental Research·Benita L McVickerCarol A Casey
Mar 28, 2008·Traffic·Floriane FaustMichael Schrader
Jun 14, 2008·Biology of the Cell·Catherine DecaensDoris Cassio
May 12, 2004·Biochemical Pharmacology·Courtney S SchaffertDean J Tuma
May 23, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yasuhiro YamazakiJunko Sugatani

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