Apical serine protease activity is necessary for assembly of a high-resistance renal collecting duct epithelium

Acta Physiologica
M SteensgaardB L Jensen

Abstract

We hypothesized that the serine protease prostasin is necessary for differentiation of a high-resistance renal collecting duct epithelium governed by glucocorticoid. Postnatal rat kidney and adult human kidney was used to study the expression and localization of prostasin. The murine collecting duct cell line (M-1) was cultured in Snapwell inserts to investigate the significance of prostasin for the development of transepithelial electrical resistance (TER). In the cortex and medulla of rat kidney, prostasin mRNA and protein increased significantly between birth and weaning (day 21) and was detected in collecting ducts. Immunoreactive prostasin was associated with collecting ducts and loops of Henle in human kidney. In rat, adrenalectomy at day 10 had no effect on prostasin mRNA level in kidney at day 20. Cultured M-1 cells exhibited parallel increases in prostasin mRNA, protein and TER 5 days after seeding. Apical addition of the serine protease inhibitor aprotinin to M-1 cell cultures inhibited development of TER and led to aberrant localization of E-cadherin. This effect was mimicked by the protease inhibitor nafamostat. Apical addition of phospholipase C to cleave glycosylphosphatidylinositol (GPI) anchors released prostasi...Continue Reading

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Citations

Apr 7, 2011·Clinical and Experimental Pharmacology & Physiology·Per SvenningsenBoye L Jensen
Jul 5, 2011·Annual Review of Cell and Developmental Biology·Roman Szabo, Thomas H Bugge
Jun 11, 2014·Acta Physiologica·P SvenningsenB L Jensen
Aug 13, 2011·Nephron. Physiology·G JacquilletR J Unwin
Jul 26, 2014·Journal of the American Society of Nephrology : JASN·Rikke M ZacharPer Svenningsen
Jun 15, 2011·Current Opinion in Nephrology and Hypertension·Per SvenningsenOle Skøtt
Dec 15, 2010·The Journal of Biological Chemistry·Stine FriisLotte K Vogel
Mar 16, 2021·Acta Physiologica·Per Svenningsen

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