PMID: 9531553May 16, 1998Paper

Apical vesicles bearing inositol 1,4,5-trisphosphate receptors in the Ca2+ initiation site of ductal epithelium of submandibular gland

The Journal of Cell Biology
M Yamamoto-HinoKatsuhiko Mikoshiba

Abstract

In polarized epithelial cells, agonists trigger Ca2+ waves and oscillations. These patterns may be caused by the compartmentalization of inositol 1,4,5-trisphosphate (IP3)-sensitive Ca2+ pools into specific regions. We have investigated the relationship between the distribution of IP3 receptors (IP3Rs) and the spatiotemporal pattern of Ca2+ signaling in the duct cells of the rat submandibular gland (SMG). Using immunofluorescence, although labeling was somewhat heterogeneous, the IP3Rs were colocalized to the apical pole of the duct cells. Immunoelectron microscopy identified small apical vesicles bearing IP3R2 in some types of duct cells. Real-time confocal imaging of intact ducts demonstrated that, after carbachol stimulation, an initial Ca2+ spike occurred in the apical region. Subsequently, repetitive Ca2+ spikes spread from the apical to the middle cytoplasm. These apical Ca2+ initiation sites were found only in some "pioneer cells," rather than in all duct cells. We performed both Ca2+ imaging and immunofluorescence on the same ducts and detected the strongest immunosignals of IP3R2 in the Ca2+ initiation sites of the pioneer cells. The subcellular localization and expression level of IP3Rs correlated strongly with the sp...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Sep 1, 1991·The American Journal of Physiology·J P Dehaye, R J Turner
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P VolpeD P Lew
Jun 1, 1994·Current Opinion in Neurobiology·T FuruichiK Mikoshiba
Jun 16, 1995·The Journal of Biological Chemistry·T MonkawaK Mikoshiba
Jul 1, 1994·Physiological Reviews·T PozzanJ Meldolesi
Jan 28, 1993·Nature·M J Berridge
Nov 1, 1996·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T SugiyamaM Hasegawa
Mar 1, 1997·The Journal of Physiology·M J Berridge

❮ Previous
Next ❯

Citations

Jan 18, 2003·Cellular Signalling·Kirill KiselyovShmuel Muallem
Dec 31, 2005·Molecular and Cellular Biology·Mizuki GotoSakuhei Fujiwara
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jun YangJ Kevin Foskett
Sep 23, 1998·The Journal of Cell Biology·J Meldolesi, T Pozzan
Dec 17, 2014·Biochimica Et Biophysica Acta·Tamara VervloessemJan B Parys
Apr 20, 2011·Biochemical and Biophysical Research Communications·Takahiro FujimotoSenji Shirasawa
Mar 23, 2011·Biochemical and Biophysical Research Communications·Takahiro FujimotoSenji Shirasawa
Apr 5, 2011·Biochemical and Biophysical Research Communications·Takahiro FujimotoSenji Shirasawa
Aug 2, 2000·The Journal of Physiology·K E FogartyP Thorn
Feb 26, 2005·Biology of the Cell·Caroline CruttwellJean-Pierre Mauger
Sep 16, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Santanu Banerjee, Gaiti Hasan
Apr 29, 2014·Biochimica Et Biophysica Acta·Haidar AklGeert Bultynck
Sep 3, 2005·Biochemical and Biophysical Research Communications·Haruo Takemura, Yoshiyuki Horio
Feb 5, 2000·Current Biology : CB·D ThomasM D Bootman
Jun 12, 1999·Biochemical and Biophysical Research Communications·H TakemuraA Segawa
Jun 9, 2000·Annual Review of Physiology·J F Kidd, P Thorn
Feb 11, 2000·Cell Calcium·C W TaylorS A Morris
Feb 15, 2002·Advances in Dental Research·D M ShinS Muallem
Mar 19, 2014·Cell Calcium·Paula J BartlettAndrew P Thomas
Nov 13, 2004·The Journal of Biological Chemistry·Michelle Ferreri-JacobiaJ Kevin Foskett
Nov 4, 2006·The Journal of Physiological Sciences : JPS·Yutaka EndoMasumi Inoue
Jun 28, 2002·Physiological Reviews·Michael C Ashby, Alexei V Tepikin
Jul 11, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Paula GioinoJuan P Ianowski
Feb 12, 2019·Archives of Oral Biology·Atsara RawangwongWiphawi Hipkaeo
Oct 1, 2005·Science·Akira FutatsugiKatsuhiko Mikoshiba

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.