Apigenin and apigeninidin isolates from the Sorghum bicolor leaf targets inflammation via cyclo-oxygenase-2 and prostaglandin-E2 blockade

International Journal of Rheumatic Diseases
Samira B L MakanjuolaAdedoyin Dosunmu

Abstract

This study evaluated the anti-inflammatory properties of a species of Sorghum bicolor leaf (SBL) grown in West Africa. Cyclo-oxygenase (COX)-2:COX-1 selectivity assay was carried out by plating isolated peripheral blood mononuclear cells in culture medium with specific SBL fractions: crude extract (J), ethyl-acetate (JE) and aqueous (JA); secondary compounds from JE (JE5, JE6, JE7 and JE8); purified (P9) and semi-purified (P8) compounds from JE5 at 5-200 μg/mL for 1 hour. Test compounds and controls ibuprofen (50 μmol/L) and CAY10404 (1 μmol/L; 10 μmol/L) were added to two sets of plates, one without lipopolyshaccharide (LPS) and the other with LPS (1 μg/mL) for 24 hour. COX-2IC50 :COX-1IC50 ratio represented 50% inhibition of the activity of COX-2 to that of COX-1 using ibuprofen as control. In separate experiments the supernatant of P8 and P9-treated fractions of SBL and controls were plated with RAW 264.7 macrophage cells to measure prostaglandin (PG)-E2 production and cell proliferation activity. JA fraction of SBL had the highest ratio of COX-2IC50 :COX-1IC5041.214 whereas JE had the lowest ratio COX-2IC50 :COX-1IC501.161 . Interestingly, JE5 derived from JE showed a ratio of COX-2IC50 :COX-1IC500.495 while P8 derived from...Continue Reading

References

Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J A MitchellJ R Vane
Jan 1, 1996·Annual Review of Immunology·M FeldmannR N Maini
Sep 1, 1996·British Journal of Rheumatology·D LloydsM B Hallett
Dec 10, 1999·Journal of Agricultural and Food Chemistry·M P KähkönenM Heinonen
Nov 26, 1999·Biochimica Et Biophysica Acta·H Schachter
Jan 12, 2000·Mayo Clinic Proceedings·E L Matteson
Jan 29, 2000·Rheumatology International·H S OztürkI Durak
Dec 20, 2002·Nature·Carl Nathan
Oct 30, 2003·Journal of Agricultural and Food Chemistry·Joseph M AwikaLuis Cisneros-Zevallos
Jul 9, 2004·Journal of Agricultural and Food Chemistry·Joseph M AwikaRalph D Waniska
Nov 13, 2004·Journal of Pharmacological Sciences·Hyun Pyo KimSam Sik Kang
Apr 3, 2008·European Journal of Immunology·Annie George-ChandyKristina Eriksson
Jul 25, 2008·Nature·Ruslan Medzhitov
Mar 24, 2009·Journal of Applied Microbiology·P BasavarajuH J L Jørgensen
Aug 3, 2010·Journal of Medicinal Food·Amy BurdettePhillip Greenspan
Feb 17, 2011·Journal of Agricultural and Food Chemistry·A P Polycarpe KayodéSusanne Siebenhandl-Ehn
Apr 12, 2012·Journal of Food Science·Bhimalingeswarappa GeeraJoseph M Awika
May 29, 2015·Journal of Basic and Clinical Physiology and Pharmacology·Solomon UmukoroAbayomi M Ajayi

❮ Previous
Next ❯

Citations

Oct 12, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Darija Šarić MustapićMirza Bojić
Nov 13, 2020·Journal of the Science of Food and Agriculture·Darshika AmarakoonSeong-Ho Lee
Sep 1, 2019·Comprehensive Reviews in Food Science and Food Safety·Yun XiongZhongxiang Fang
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Yun XiongZhongxiang Fang
May 7, 2019·Autoimmunity Reviews·Haroon KhanMichael Aschner

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here