Abstract
Due to the challenges in the control, prevention, and eradication of parasitic diseases like malaria, there is an urgent need to discover new therapeutic agents. Plant-derived medicines may open new ways in the field of antiplasmodial therapy. This study is aimed to investigate the toxicity and in vivo antiplasmodial activity of apigenin, a dietary flavonoid. Apigenin cytotoxicity was investigated on Huh7 cell line, brine shrimp (Artemia salina) larva, and human red blood cells. In vivo toxicity of apigenin was assessed by metabolomics approaches. Apigenin exhibited significant suppression of parasitemia in a dose-dependent manner; it suppressed Plasmodium berghei growth by 69.74%, 50.3%, and 49.23% at concentrations of 70, 35, and 15 mg/kg/day, respectively. The IC50 value for apigenin after 24 hr exposure to Huh7 cells was 225 μg/ml. Apigenin did not show noticeable toxicity on A. salina and also on the membrane integrity of red blood cells. After 24 hr exposure of mice to apigenin, alterations were seen in the metabolism of glucocorticoids and mineralocorticoids, bile acid metabolism (alternative pathway), sulfur metabolism, bile acid metabolism, metabolism of estrogens and androgens, cholesterol catabolism, and biosynthesis...Continue Reading
References
Oct 1, 1990·The Journal of Steroid Biochemistry and Molecular Biology·A R Ibrahim, Y J Abul-Hajj
Jan 1, 1997·Life Sciences·R DaiF K Friedman
May 26, 2001·Annual Review of Nutrition·C S YangH L Newmark
Sep 22, 2001·The American Journal of Clinical Nutrition·R J NijveldtP A van Leeuwen
Jun 18, 2002·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Inga KöhlerEckart Eich
Oct 25, 2002·Journal of the American Dietetic Association·Laura SampsonMartijn B Katan
Oct 7, 2004·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Angéline GradolattoMarie-Chantal Canivenc-Lavier
Mar 30, 2006·Antimicrobial Agents and Chemotherapy·Deniz TasdemirPeter Rüedi
Jan 5, 2007·Nucleic Acids Research·David S WishartLori Querengesser
May 1, 1982·Planta medica·B N MeyerJ L McLaughlin
Jun 1, 1949·The Annals of Applied Biology·D J FINNEY
Aug 20, 2008·BMC Research Notes·Adele M Lehane, Kevin J Saliba
Mar 23, 2010·Pharmaceutical Research·Sanjeev Shukla, Sanjay Gupta
Jun 12, 2010·The Journal of Nutritional Biochemistry·Wei LiXingjia Wang
Sep 25, 2012·Free Radical Biology & Medicine·Claude A Piantadosi, Hagir B Suliman
Feb 2, 2013·Biological & Pharmaceutical Bulletin·Eri HasegawaSusumu Yamato
Aug 8, 2013·Parasitology Research·Hadi SangianAli Ramazani
Dec 18, 2013·PloS One·Sarah C PreissnerSaskia Preissner
Mar 26, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lindie Schloms, Amanda C Swart
Jan 1, 2013·Computational and Structural Biotechnology Journal·Jörg BartelFabian J Theis
Oct 2, 2014·Methods in Molecular Biology·Bowei XiDaniel Raftery
Apr 19, 2015·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Somayeh RajabiTahereh Naji
May 8, 2015·International Journal for Parasitology. Drugs and Drug Resistance·Liliane Abodo OnambeleMakoah Nigel Aminake
May 23, 2015·Chemistry & Biology·Katharina Eick, Georg Pohnert
Jun 4, 2015·Biochimica Et Biophysica Acta·Igor A GakSilvana A Andric
Aug 16, 2015·Inflammation·Rajeshwari H PatilS Chidananda Sharma
Oct 22, 2015·Neural Regeneration Research·Madhuri VenigallaGerald Münch
Nov 4, 2015·Neurochemistry International·Madhuri VenigallaGerald Münch
Feb 11, 2016·PLoS Neglected Tropical Diseases·Fernanda Fonseca-SilvaElmo E Almeida-Amaral
May 19, 2016·Expert Opinion on Pharmacotherapy·Nicholas J White
Nov 5, 2016·Phytotherapy Research : PTR·Boram KimHye Jin Jung
Feb 6, 2017·The Lancet Infectious Diseases·Mallika ImwongArjen M Dondorp
Mar 31, 2017·Drug Development and Industrial Pharmacy·Zhenhai ZhangHuixia Lv
Apr 30, 2017·Cell Biology and Toxicology·Michelle CormierLuc J Martin
May 4, 2017·Phytotherapy Research : PTR·Paolino NinfaliEmanuele-Salvatore Scarpa
Jun 2, 2017·Experimental and Therapeutic Medicine·Kun ZhangXing Jin
Aug 16, 2017·International Journal of Antimicrobial Agents·Ohud Fallatah, Elias Georges