Apixaban decreases brain thrombin activity in a male mouse model of acute ischemic stroke

Journal of Neuroscience Research
Doron BushiDavid Tanne

Abstract

Factor Xa (FXa) plays a critical role in the coagulation cascade by generation of thrombin. During focal ischemia thrombin levels increase in the brain tissue and cause neural damage. This study examined the hypothesis that administration of the FXa inhibitor, apixaban, following focal ischemic stroke may have therapeutic potential by decreasing brain thrombin activity and infarct volume. Male mice were divided into a treated groups that received different doses of apixaban (2, 20, 100 mg/kg administered I.P.) or saline (controls) immediately after blocking the middle cerebral artery (MCA). Thrombin activity was measured by a fluorescence assay on fresh coronal slices taken from the mice brains 24 hr following the MCA occlusion. Infarct volume was assessed using triphenyltetrazolium chloride staining. A high dose of apixaban (100 mg/kg) significantly decreased thrombin activity levels in the ipsilateral hemisphere compared to the control group (Slice#5, p = .016; Slice#6, p = .016; Slice#7, p = .016; Slice#8, p = .036; by the nonparametric Mann-Whitney test). In addition, treatment with apixaban doses of both 100 mg/kg (32 ± 8% vs. 76 ± 7% in the treatment vs. control groups respectively; p = .005 by the nonparametric Mann-Whit...Continue Reading

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Citations

Jul 21, 2020·Research and Practice in Thrombosis and Haemostasis·Edward L G PryzdialMarc Horwitz
Jan 20, 2019·Journal of Thrombosis and Haemostasis : JTH·Michael R SutherlandEdward L G Pryzdial
Aug 10, 2019·Neural Regeneration Research·Shany G Gofrit, Efrat Shavit-Stein
Dec 10, 2020·Journal of Neuroscience Research·Ekaterina MindelEfrat Shavit-Stein
Jan 5, 2021·Cellular and Molecular Neurobiology·Hui CaoWenting Song

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