PMID: 9424734Jan 10, 1998Paper

Aplastic anemia: a model for its induction by oral and subcutaneous benzene in rats

Sangre
E Barrera EscorciaA Vargas Vera

Abstract

To set up the experimental conditions to induce aplastic anaemia in rats by oral and subcutaneous administration of benzene. 6 groups with 6 male Wistar rats weighting 150 g each were formed. Each group with different conditions; 3 of them as experimental groups: 1) ES group (benzene 2 mL/Kg supplied subcutaneously), 2) EOI and 3) EOII groups (benzene 1.14 and 2 mL/Kg supplied orally); and 3 groups as control: 4) TS and 5) TO groups (supplied only with the vehicle subcutaneously. and orally, respectively) and 6) C group without treatment. Benzene was supplied during four weeks. Blood counts were done at 0, 15 and 30 days of treatment. EOI and EOII treatment was interrupted because of a severe damage to rats and the other groups except TO group continued until 60 days. When the treatment ended haematological determinations continued for 60 days every two weeks including osmotic fragility test and bone marrow smears in order to observe permanent changes. The rats treated with benzene 4 weeks showed a reduction in concentration of haemoglobin, bleeding of nasal and gastric mucosae, thrombocytopenia, microcytosis and macrocytosis in EOI and EOII groups respectively. The animals treated with benzene 60 days (ES) showed persistent re...Continue Reading

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