APM_GUI: analyzing particle movement on the cell membrane and determining confinement.

BMC Biophysics
Silvia A MenchónCarlos G Dotti

Abstract

Single-particle tracking is a powerful tool for tracking individual particles with high precision. It provides useful information that allows the study of diffusion properties as well as the dynamics of movement. Changes in particle movement behavior, such as transitions between Brownian motion and temporary confinement, can reveal interesting biophysical interactions. Although useful applications exist to determine the paths of individual particles, only a few software implementations are available to analyze these data, and these implementations are generally not user-friendly and do not have a graphical interface,. Here, we present APM_GUI (Analyzing Particle Movement), which is a MatLab-implemented application with a Graphical User Interface. This user-friendly application detects confined movement considering non-random confinement when a particle remains in a region longer than a Brownian diffusant would remain. In addition, APM_GUI exports the results, which allows users to analyze this information using software that they are familiar with. APM_GUI provides an open-source tool that quantifies diffusion coefficients and determines whether trajectories have non-random confinements. It also offers a simple and user-friendl...Continue Reading

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Citations

Apr 13, 2013·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Carol B JohnsonAndreas Holzenburg
Oct 22, 2014·European Biophysics Journal : EBJ·Dipak MainaliEmily A Smith
Nov 8, 2018·Biochimica Et Biophysica Acta. Biomembranes·Qiaochu Zhu, Emily A Smith

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Methods Mentioned

BETA
fluorescence microscopy

Software Mentioned

MatLab Command Windows
SPT
Particle Tracker
GUI
Imaris
AMP
APM
ImageJ
Imaris Track
MatLab scripts

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