Sep 1, 1991

ApoA-I secretion by rabbit intestinal mucosa cell cultures

T L Carlson, B A Kottke


Lipid and apolipoprotein (apo) A-I concentrations in different density fractions of New Zealand White (NZW) and Watanabe (WHHL) rabbit plasma were studied. Aside from the low plasma apoA-I and high density lipoprotein (HDL) cholesterol levels in WHHL rabbits, the distribution of apoA-I was also different between the two rabbits. ApoA-I was concentrated in both the HDL2 and HDL3 fractions of NZW rabbits but was found primarily in the HDL3 fraction of WHHL rabbits. ApoA-I secretion in these two rabbits was further studied in vitro by using intestinal and hepatocyte cell cultures. ApoA-I secretion was highest from cultures of the duodenum and the proximal end of the jejunum; whereas, cell cultures of the distal end of the small intestine secreted very little apoA-I into the medium. Intestinal cell cultures from WHHL rabbits secreted less, but significant amounts of, apoA-I compared to that of NZW rabbits. ApoA-I was most concentrated in the density range of 1.12-1.21 (HDL3) fraction in medium containing 10% fetal calf serum (FCS). Serum-free medium promoted apoA-I secretion by intestinal cell cultures that was mostly found in the d greater than 1.21 (lipoprotein-deficient) fraction. Hepatocytes isolated from the same rabbits by co...Continue Reading

  • References7
  • Citations1


  • References7
  • Citations1

Mentioned in this Paper

Structure of Calf of Leg
Malignant Neoplasm of Jejunum
HDL-Cholesterol Subclass 3 Measurement
Apolipoprotein Measurement
Huntington DISEASE-LIKE 3 (Disorder)
Small Intestinal Wall Tissue
Cattle calf (organism)
Cell Culture Techniques
Fresh Frozen Plasma

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