Apocynin attenuates testicular ischemia-reperfusion injury in rats

Journal of Pediatric Surgery
Tarik Emre SenerGoksel Sener

Abstract

This study was designed to examine the possible protective effect of apocynin, a NADPH oxidase inhibitor, against torsion/detorsion (T/D) induced ischemia/reperfusion (I/R) injury in testis. Male Wistar albino rats were divided into sham-operated control, and either vehicle, apocynin 20mg/kg- or apocynin 50mg/kg-treated T/D groups. In order to induce I/R injury, left testis was rotated 720° clockwise for 4 hours (torsion) and then allowed reperfusion (detorsion) for 4 hours. Left orchiectomy was done for the measurement of tissue malondialdehyde (MDA), glutathione (GSH) levels, myeloperoxidase (MPO) activity, and luminol, lucigenin, nitric oxide (NO) and peroxynitrite chemiluminescences (CL). Testicular morphology was examined by light microscopy. I/R caused significant increases in tissue luminol, lucigenin, nitric oxide and peroxynitrite CL demonstrating increased reactive oxygen and nitrogen metabolites. As a result of increased oxidative stress tissue MPO activity, MDA levels were increased and antioxidant GSH was decreased. On the other hand, apocynin treatment reversed all these biochemical indices, as well as histopathological alterations that were induced by I/R. According to data, although lower dose of apocynin tended...Continue Reading

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Citations

Apr 30, 2016·Oxidative Medicine and Cellular Longevity·Letteria MinutoliDomenica Altavilla
Oct 12, 2017·Acta Cirúrgica Brasileira·Aris FakouriPejman Mortazavi
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Nov 12, 2021·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Selim DemirAhmet Mentese

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