Apocynin inhibits the upregulation of TGF-β1 expression and ROS production induced by TGF-β in skeletal muscle cells

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Johanna AbrigoClaudio Cabello-Verrugio

Abstract

Pure apocynin, which can be traditionally isolated and purified from several plant species such as Picrorhiza kurroa Royle ex Benth (Scrophulariaceae), acts as an inhibitor of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) activity inhibiting its production of reactive oxygen species (ROS). Transforming growth factor type beta 1 (TGF-β1) is a growth factor that produces inhibition of myogenesis, diminution of regeneration and induction of atrophy in skeletal muscle. The typical signalling that is activated by TGF-β involves the Smad pathway. To evaluate the effect of TGF-β and the effect of apocynin on TGF-β1 expression in skeletal muscle cells. Controlled laboratory study. In vitro assays were performed with C2C12 cells incubated with TGF-β1 in presence or absence of apocynin (NOX inhibitor), SB525334 (TGF-β-receptor I inhibitor), or chelerythrine (PKC inhibitor). TGF-β1 and atrogin-1 expression was evaluated by RT-qPCR and/or ELISA; Smad3 phosphorylation by western blot; Smad4 nuclear translocation by indirect immunofluorescence; and ROS levels by DCF probe fluorescent measurements. We show that myoblasts respond to TGF-β1 by increasing its own gene expression in a time- and dose-dependent fashion which was...Continue Reading

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Citations

Nov 7, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Samik BinduMahesh P Gupta
May 23, 2018·International Journal of Environmental Research and Public Health·Joanna WieczfinskaRafal Pawliczak
Oct 8, 2019·Antioxidants & Redox Signaling·Carlos Henríquez-OlguínThomas E Jensen
Dec 12, 2018·Cell Communication and Signaling : CCS·Xiaofan YangZhenbing Chen
Feb 14, 2021·Cell Biochemistry and Biophysics·Ling PengChangming Wang

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