ApoE synthesis in human neuroblastoma cells

Neurobiology of Disease
L Dupont-WalloisM L Caillet-Boudin

Abstract

Apolipoprotein E (apoE) is associated with the two hallmarks of Alzheimer's disease: A beta deposits and neurofibrillary tangles. ApoE synthesis was detected in astrocytes by in situ hybridization but was not detected in neurons. Nevertheless, different studies on apoE immunoreactivity reported the presence of apoE in neurons of Alzheimer, control, and necrosis pontisubicular brains. In this study, we addressed the question of potential synthesis of apoE in neurons and its possible involvement in or in response to pathological conditions. To this purpose, we have studied human neuronal cell lines (SY 5Y and Kelly cells) originating from neuroblastoma. Using monoclonal and polyclonal antibodies, a 32-kDa band was detected in SY 5Y and Kelly cells, before and after NGF differentiation. Two-dimensional gel electrophoresis analysis showed a typical profile of apoE spots resolved to the exact isoelectric points. By reverse transcription-polymerase chain reaction experiments, we demonstrated the presence of apoE mRNA in these cell lines. SY 5Y cells synthesized the apoE3 variant, whereas Kelly cells expressed both apoE3 and apoE4 isoforms, corroborating the two-dimensional gel results. These results suggested that apoE synthesis coul...Continue Reading

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Citations

Feb 23, 2010·BMC Neuroscience·David A ElliottBrett Garner
May 12, 2011·International Journal of Alzheimer's Disease·Valérie LeducJudes Poirier
Mar 29, 2006·Proceedings of the National Academy of Sciences of the United States of America·Robert W MahleyYadong Huang
Aug 27, 2003·Proceedings of the National Academy of Sciences of the United States of America·Faith M HarrisYadong Huang
Dec 14, 2005·Proceedings of the National Academy of Sciences of the United States of America·Shengjun ChangYadong Huang
Mar 13, 2009·Neurobiology of Aging·David A ElliottBrett Garner
Oct 31, 2003·The Journal of Biological Chemistry·Faith M HarrisYadong Huang
Mar 22, 2003·Stroke; a Journal of Cerebral Circulation·Kazuko AokiYoshihiro Wakayama
May 10, 2008·Expert Review of Proteomics·Nicolas SergeantLuc Buée
Sep 14, 2017·Journal of Integrative Bioinformatics·Vladimir N BabenkoNatalia N Kudryavtseva

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