APOEε4 increases trauma induced early apoptosis via reducing delayed rectifier K(+) currents in neuronal/glial co-cultures model

Experimental Cell Research
Ligang ChenLi Kuai

Abstract

Traumatic brain injury (TBI) is a commonly encountered emergency and severe neurosurgical injury. Previous studies have shown that the presence of the apolipoprotein E (APOE) ε4 allele has adverse outcomes across the spectrum of TBI severity. Our objective was to evaluate the effects of APOE alleles on trauma induced early apoptosis via modification of delayed rectifier K(+) current (Ik(DR)) in neuronal/glial co-cultures model. An ex vivo neuronal/glial co-cultures model carrying individual APOE alleles (ε2, ε3, ε4) of mechanical injury was developed. Flow cytometry and patch clamp recording were performed to analyze the correlations among APOE genotypes, early apoptosis and Ik(DR). We found that APOEε4 increased early apoptosis at 24h (p<0.05) compared to the ones transfected with APOEε3 and APOEε2. Noticeably, APOEε4 significantly reduced the amplitude of the Ik(DR) at 24h compared to the APOEε3 and APOEε2 (p<0.05) which exacerbate Ca(2+) influx. This indicates a possible effect of APOEε4 on early apoptosis via inhibiting Ik(DR) following injury which may adversely affect the outcome of TBI.

References

Jan 1, 1990·Cold Spring Harbor Symposia on Quantitative Biology·P AndersenJ F Storm
Mar 3, 1994·The New England Journal of Medicine·S A Lipton, P A Rosenberg
May 28, 1993·Annals of the New York Academy of Sciences·S J GibbonsR J Miller
Jul 14, 1998·Nature Medicine·C R Plata-Salamán
May 20, 2003·Journal of Lipid Research·Robert M DeKroonWarren J Strittmatter
Sep 23, 2003·Critical Care Medicine·Mary E KerrDonald W Marion
Jul 17, 2004·The European Journal of Neuroscience·Marusa HribarLorenza Eder-Colli
Apr 22, 2005·Intensive Care Medicine·Eduardo MiñambresMarcos López-Hoyos
May 11, 2006·The Journal of Membrane Biology·E D BurgJ X-J Yuan
Jul 6, 2006·Trends in Biochemical Sciences·Danny M HattersKarl H Weisgraber
Sep 12, 2006·Intensive Care Medicine·Maria Angeles BallesterosEduardo Miñambres
Sep 26, 2006·Neuroscience Letters·Yong JiangYingjiang Gu
Mar 28, 2008·Journal of Neurotrauma·Eduardo MiñambresMarcos López-Hoyos
Aug 1, 2008·Chinese Journal of Traumatology = Zhonghua Chuang Shang Za Zhi·Xiao-chuan Sun, Yong Jiang
Jan 2, 2010·Neurosurgical Focus·Efthimios DardiotisGeorgios M Hadjigeorgiou

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

ApoE Phenotypes

Apolipoprotein E (APOE) is a protein involved in fat metabolism and associated with the pathogenesis of Alzheimer's disease and cardiovascular disease. Here is the latest research on APOE phenotypes.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology
Emma De FeoStefania Boccia
Journal of Neurology, Neurosurgery, and Psychiatry
Kristiina RannikmäeCathie L M Sudlow
Clinical Nephrology
Rebecca Kurnik SeshasaiMark J Sarnak
© 2021 Meta ULC. All rights reserved