Apogossypolone, a novel inhibitor of antiapoptotic Bcl-2 family proteins, induces autophagy of PC-3 and LNCaP prostate cancer cells in vitro.

Asian Journal of Andrology
Xian-Qing ZhangDao-Cheng Wu

Abstract

Limited treatment options are available for aggressive prostate cancer. Gossypol has been reported to have a potent anticancer activity in many types of cancer. It can increase the sensitivity of cancer cells to alkylating agents, diminish multidrug resistance and decrease metastasis. Whether or not it can induce autophagy in cancer cells has not yet been determined. Here we investigated the antiproliferative activity of apogossypolone (ApoG2) and (-)-gossypol on the human prostate cancer cell line PC3 and LNCaP in vitro. Exposure of PC-3 and LNCaP cells to ApoG2 resulted in several specific features characteristic of autophagy, including the appearance of membranous vacuoles in the cytoplasm and formation of acidic vesicular organelles. Expression of autophagy-associated LC3-II and beclin-1 increased in both cell lines after treatment. Inhibition of autophagy with 3-methyladenine promoted apoptosis of both cell types. Taken together, these data demonstrated that induction of autophagy could represent a defense mechanism against apoptosis in human prostate cancer cells.

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Citations

Jun 20, 2012·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Xiaoge NiuSenming Wang
May 6, 2015·Seminars in Cancer Biology·Ramzi M MohammadAsfar S Azmi
Feb 13, 2015·Molecular Medicine Reports·Wenhua ZhanXiaofeng Huang
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May 27, 2017·Current Medicinal Chemistry·Yun ZengDaocheng Wu
Apr 4, 2014·Organic & Biomolecular Chemistry·Fang-Wu WangJun-Ying Miao
May 18, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Yong-Hua ZhanXian-Qing Zhang
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May 5, 2020·Current Drug Targets·Fatemeh Forouzanfar, Seyed Hadi Mousavi
Feb 6, 2021·Nutrition Research and Practice·Sang-Han Lee, Yoon-Jin Lee

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