Apolipoprotein A-I metabolism in cynomolgus monkey. Identification and characterization of beta-migrating pools

Arteriosclerosis : an Official Journal of the American Heart Association, Inc
G W Melchior, C K Castle

Abstract

Fresh plasma from control (C) and hypercholesterolemic (HC) cynomolgus monkeys was analyzed by agarose electrophoresis-immunoblotting with antibody to cynomolgus monkey apolipoprotein (apo) A-I. Two bands were evident on the autoradiogram: an alpha-migrating band (high density lipoprotein) and a beta-migrating band that comigrated exactly with cynomolgus monkey low density lipoprotein (LDL). The presence of beta-migrating apo A-I in the plasma of these monkeys was confirmed by Geon-Pevikon preparative electrophoresis, crossed immunoelectrophoresis, and isotope dilution studies in which radiolabeled apo A-I was found to equilibrate also with alpha- and beta-migrating pools of apo A-I in the plasma. Subfractionation of C and HC plasma by agarose column chromatography (Bio-Gel A-0.5M and A-15M) followed by agarose electrophoresis-immunoblotting indicated that the beta-migrating apo A-I in C was relatively homogeneous and eluted with proteins of Mr approximately 50 kD [apo A-I(50 kD)], whereas two beta-migrating fractions were identified in HC, one that eluted with the 50-kD proteins, and the other that eluted in the LDL Mr range [apo A-I(LDL)]. The apo A-I(LDL) was precipitated by antibody to cynomolgus monkey apo B. The apo A-I(5...Continue Reading

References

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Citations

Mar 12, 1990·Biochimica Et Biophysica Acta·G W MelchiorK R Marotti
Nov 27, 1991·Biochimica Et Biophysica Acta·M E PapeG W Melchior
Apr 19, 1996·Biochimica Et Biophysica Acta·A BarransX Collet
Jun 1, 1992·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·K R MarottiG W Melchior
Jun 5, 1998·The American Journal of Physiology·C K CastleG W Melchior

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