Apolipoprotein A-I Supports MSCs Survival under Stress Conditions.

International Journal of Molecular Sciences
Svetlana MiroshnichenkoAnastasiya Solovieva

Abstract

Clinical trials have shown the safety of mesenchymal stem/stromal cells (MSCs) transplantation, but the effectiveness of these treatments is limited. Since, transplanted MSCs will undergo metabolic disturbances in the bloodstream, we investigated the influence of blood plasmas of type 2 diabetes (T2D) patients on MSCs viability and examined whether apolipoprotein A-I (apoA-I) could protect cells from stressful conditions of serum deprivation (SD), hypoxia, and elevated concentrations of reactive oxygen species (ROS). ApoA-I exhibits anti-inflammatory, immune activities, improves glycemic control, and is suitable for T2D patients but its influence on MSCs remains unknown. For the first time we have shown that apoA-I decreases intracellular ROS and supports proliferative rate of MSCs, thereby increasing cell count in oxidation conditions. ApoA-I did not influence cell cycle when MSCs were predominantly in the G0/G1 phases under conditions of SD/hypoxia, activated proliferation rapidly, and reduced apoptosis during MSCs transition to the oxygenation or oxidation conditions. Finally, it was found that the blood plasma of T2D individuals had a cytotoxic effect on MSСs in 39% of cases and had a wide variability of antioxidant propert...Continue Reading

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Citations

Feb 10, 2021·International Journal of Molecular Sciences·Christian Sávio-SilvaÉrika Bevilaqua Rangel
Jul 11, 2021·Cell Biology International·Vijayendran GovindasamySoon-Keng Cheong

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Methods Mentioned

BETA
gel filtration
Infrared Spectroscopy
Assay
flow cytometry
electrophoresis
fluorescence microscopy

Software Mentioned

Origin Pro
ImageJ
Cell Activision
Kaluza
OriginPro
STATISTICA

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis