Apolipoprotein B is conformationally flexible but anchored at a triolein/water interface: a possible model for lipoprotein surfaces.

Proceedings of the National Academy of Sciences of the United States of America
Libo WangDonald M Small

Abstract

Apolipoprotein B (apoB) is one of a unique group of proteins that form and bind to fat droplets, stabilize the emulsified fat, and direct their metabolism. ApoB, secreted on lipoproteins (emulsions), remains bound during lipid metabolism yet exhibits conformational flexibility. It has amphipathic beta-strand (AbetaS)-rich domains and amphipathic alpha-helix (AalphaH)-rich domains. We showed that two consensus AbetaS peptides of apoB bound strongly to hydrophobic interfaces [triolein/water (TO/W) and dodecane/water], were elastic, and were not pushed off the interface when the surface was compressed. In contrast, an AalphaH peptide modeling helical parts of apoB was forced off the TO/W interface by compression and readsorbed when the interface was expanded. In this report, the surface behavior of apoB-100 was studied at the TO/W interface. Solubilized apoB lowered the interfacial tension of TO/W in a concentration-dependent fashion. At equilibrium tension, if the surface was compressed, part of apoB was pushed off but quickly readsorbed when the surface was expanded. Even when the surface area was compressed by approximately 55%, part of the apoB molecule remained bound. The maximum surface pressure that apoB could withstand wit...Continue Reading

References

Jun 1, 1975·The Journal of Clinical Investigation·O Faergeman, R J Havel
Mar 1, 1991·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·J de GraafA F Stalenhoef
Jan 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·C Y YangA M Gotto
Jan 1, 1986·Methods in Enzymology·M C Phillips, K E Krebs
Apr 1, 1996·Plant Physiology·A H Huang
Apr 27, 1999·Trends in Biochemical Sciences·D J Murphy, J Vance
Aug 23, 2001·Current Biology : CB·D A Brown
Oct 20, 2001·Mammalian Genome : Official Journal of the International Mammalian Genome Society·X LuA R Kimmel
Jul 10, 2004·Structure·Sabrina J HickenbottomJames H Hurley

❮ Previous
Next ❯

Citations

Sep 18, 2008·European Biophysics Journal : EBJ·Ruth Prassl, Peter Laggner
Nov 1, 2012·Seminars in Immunopathology·M F BassendineR D G Neely
Sep 21, 2010·Applied Biochemistry and Biotechnology·Stefania BonsegnaAngelo Santino
Nov 16, 1994·Biochimica Et Biophysica Acta·F CendrinG Zaccai
Jul 18, 1996·Biochimica Et Biophysica Acta·O M LardinoisP G Rouxhet
Aug 14, 1999·Progress in Biophysics and Molecular Biology·M Zámocký, F Koller
Feb 23, 2010·Journal of Lipid Research·Juan GuevaraNatalia Valentinova Guevara
Aug 20, 2008·Journal of Lipid Research·Aubrey S LedfordRichard B Weinberg
Mar 20, 2014·PloS One·José A Fernández-HigueroJosé L R Arrondo
Mar 21, 2007·Biophysical Journal·Zhenghui Gordon JiangC James McKnight
Apr 23, 2013·Biochimica Et Biophysica Acta·Jean-David VindigniMarine Froissard
Sep 16, 2008·Biochimica Et Biophysica Acta·Shin-ya MoritaTetsurou Handa
Oct 30, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·R M CorneliusJ L Brash
Dec 7, 2006·Journal of Lipid Research·Marcel M W SmolenaarsDick J Van der Horst
Apr 12, 2015·Journal of Lipid Research·Su Duy NguyenKatariina Öörni
Jan 14, 2017·Nature Communications·Sudipta MondalEhud Gazit
Sep 26, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Pia DavidssonGermán Camejo
Jan 17, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Matthew A MitscheDonald M Small
May 4, 2021·The Journal of Physical Chemistry. B·Mona MirheydariEdgar E Kooijman
Sep 30, 2017·ACS Applied Materials & Interfaces·Qingqing ChengDavid L Kaplan
Dec 24, 2021·The Journal of Physical Chemistry Letters·Aline CisseJudith Peters

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.