Apomorphine prevents myocardial ischemia/reperfusion-induced oxidative stress in the rat heart

Free Radical Biology & Medicine
Igor KhaliulinH Schwalb

Abstract

This study examined the hypothesis that low-concentration apomorphine improves postischemic hemodynamic and mitochondrial function in the isolated rat heart model by attenuating oxidation of myocardial proteins. Control and apomorphine-treated hearts were subjected to 35 min of perfusion, 25 min of normothermic global ischemia, and 60 min of reperfusion. Apomorphine (2 microM) was introduced into the perfusate for 20 min starting from the onset of reperfusion. Apomorphine significantly (p <.05) improved postischemic hemodynamic function: work index of the heart (product of LVDP and heart rate) was twice as high in apomorphine-treated hearts compared to controls at the end of reperfusion (p <.01). After isolation of cardiac mitochondria, the respiratory control ratio (RCR) was calculated from the oxygen consumption rate of State 3 and State 4 respiration. Apomorphine significantly improved postischemic RCR (87% of preischemic value vs. 39% in control, p <.05). Using an immunoblot technique, carbonyl content of multiple unidentified myocardial proteins (mitochondrial and nonmitochondrial) was observed to be elevated after global ischemia and reperfusion. Apomorphine significantly attenuated the increased protein oxidation at the ...Continue Reading

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Citations

Nov 23, 2006·Cardiovascular Drugs and Therapy·Eyal RamuHerzl Schwalb
Jul 4, 2012·The Journal of Physiological Sciences : JPS·Tetsuya MatsuuraMitsuyuki Ichinose
Aug 19, 2009·Antioxidants & Redox Signaling·Esther Barreiro, Sabah N A Hussain
May 9, 2006·Free Radical Biology & Medicine·Igor KhaliulinHerzl Schwalb
Jan 27, 2009·Biochimica Et Biophysica Acta·Andrew P Halestrap, Philippe Pasdois
Aug 19, 2014·Birth Defects Research. Part A, Clinical and Molecular Teratology·Hui HuJun Zhu
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Jul 28, 2010·Biochemical Society Transactions·Andrew P Halestrap

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