Apoplastic and cytoplasmic location of harpin protein Hpa1Xoo plays different roles in H2O2 generation and pathogen resistance in Arabidopsis.

Plant Molecular Biology
Suling SangHansong Dong

Abstract

Harpin proteins secreted by phytopathogenic bacteria have been shown to activate the plant defense pathway, which involves transduction of a hydrogen peroxide (H(2)O(2)) signal generated in the apoplast. However, the way in which harpins are recognized in the pathway and what role the apoplastic H(2)O(2) plays in plant defenses are unclear. Here, we examine whether the cellular localization of Hpa1(Xoo), a harpin protein produced by the rice bacterial leaf blight pathogen, impacts H(2)O(2) production and pathogen resistance in Arabidopsis thaliana. Transformation with the hpa1 (Xoo) gene and hpa1 (Xoo) fused to an apoplastic localization signal (shpa1 (Xoo)) generated h pa1 (Xoo)- and sh pa1 (Xoo)-expressing transgenic A . t haliana (HETAt and SHETAt) plants, respectively. Hpa1(Xoo) was associated with the apoplast in SHETAt plants but localized inside the cell in HETAt plants. In addition, Hpa1(Xoo) localization accompanied H(2)O(2) accumulation in both the apoplast and cytoplasm of SHETAt plants but only in the cytoplasm of HETAt plants. Apoplastic H(2)O(2) production via nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) located in the plasma membrane is a common feature of plant defenses. In SHETAt plants, H(...Continue Reading

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Citations

May 31, 2014·Molecular Plant-microbe Interactions : MPMI·Yanying ZhaoHansong Dong
May 25, 2016·Seminars in Cell & Developmental Biology·William Underwood
Jun 10, 2017·Botanical Studies·Yilan DongChunling Zhang
Sep 6, 2018·BMC Microbiology·Xuan WangHansong Dong
Jun 14, 2019·Frontiers in Plant Science·Liyuan ZhangHansong Dong
Jan 26, 2021·IScience·Ali EljebbawiJosé Manuel Estevez
Jul 18, 2021·The Plant Journal : for Cell and Molecular Biology·Xiaochen ChenHansong Dong

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