PMID: 16643782Apr 29, 2006Paper

Apoptin-induced apoptosis of human melanoma cells A375 via activation of caspase-3

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Ping DongHong-yang Li

Abstract

To study whether apoptin gene induces apoptosis in human melanoma cells A375 via the activation of caspase-3. The human melanoma cells A375 were transiently transfected with recombinant pcDNAA3 plasmid, which contained apoptin gene, and the apoptosis was measured by DNA agarose electrophoresis, RT-PCR and flow cytometry. Caspase-3 relative activity was analyzed by colorimetric assay at various time. At 48 h after transfection, the apoptin gene could induce apoptosis of human melanoma cells A375 in vitro and the mRNA of apoptin gene could be detected by RT-PCR. Caspase-3 activity began to rise at 24 h after transfection and reached the peak at 72 h. Apoptin gene can induce apoptosis in human melanoma cells A375 via active caspase-3 and the activity of caspase-3 is time-dependent.

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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