Apoptosis: A mammalian cell bioprocessing perspective

Biotechnology Advances
Antonio L Grilo, Athanasios Mantalaris

Abstract

Apoptosis is a form of programmed and controlled cell death that accounts for the majority of cellular death in bioprocesses. Cell death affects culture longevity and product quality; it is instigated by several stresses experienced by the cells within a bioreactor. Understanding the factors that cause apoptosis as well as developing strategies that can protect cells is crucial for robust bioprocess development. This review aims to a) address apoptosis from a bioprocess perspective; b) describe the significant apoptotic mechanisms linking them to the most relevant stresses encountered in bioreactors; c) discuss the design of operating conditions in order to avoid cell death; d) focus on industrially relevant cell lines; and e) present anti-apoptosis strategies including cell engineering and model-based optimization of bioprocesses. In addition, the importance of apoptosis in quality-by-design bioprocess development from clone screening to production scale are highlighted.

Citations

Aug 14, 2019·Journal of Gastrointestinal Cancer· Sohaib M, Ezhilarasan D
Apr 24, 2020·Journal of Cellular Physiology·Behnam Emamgolizadeh Gurt TapehSaeed Solali
May 18, 2020·Journal of Biomedical Materials Research. Part a·Hui CaoXiaolong Yu
Nov 7, 2019·American Journal of Respiratory Cell and Molecular Biology·Joseph D BrainJamie H Rosenblum Lichtenstein
Feb 25, 2020·Environmental Science and Pollution Research International·Nebahat YildizbayrakMelike Erkan
Oct 24, 2020·Journal of Toxicology and Environmental Health. Part a·Aline Borba SbardelottoPaulo Michel Pinheiro Ferreira
Nov 18, 2020·Biotechnology and Bioengineering·Julian SchmitzAlexander Grünberger
Dec 19, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ning ZhangJuan Du
Jan 21, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ana B V TeixeiraAndréa C Reis
Feb 18, 2021·Food Science and Technology International = Ciencia Y Tecnología De Los Alimentos Internacional·Claudia Fuente-GarciaMiguel A Sentandreu
Apr 7, 2021·Tissue Engineering. Part a·Anyesha Sarkar, Mark A Messerli
May 4, 2021·Journal of the Science of Food and Agriculture·Claudia Fuente-GarcíaMiguel Ángel Sentandreu
Jul 6, 2021·Chemical Society Reviews·Jianlei XieJong Seung Kim

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Bioreactors (ASM)

Bioreactors are important devices or systems that utilize living cells or enzymes for a chemical process. These devices carefully control and monitor the environment factors such as pH, nutrients level, and temperature, which can affect the yield in a given process. Discover the latest research here.

Related Papers

Advances in Biochemical Engineering/biotechnology
R P Singh, Mohamed Al-Rubeai
Cell Stem Cell
Daniel C Kirouac, Peter W Zandstra
Current Opinion in Biotechnology
Mohamed Al-Rubeai, R P Singh
© 2022 Meta ULC. All rights reserved