Apoptosis: a possible tactic of Haemophilus somnus for evasion of killing by bovine neutrophils?

Microbial Pathogenesis
Y F YangC J Czuprynski

Abstract

Haemophilus somnus is an important veterinary pathogen that causes respiratory disease, arthritis, septicaemia and abortion in cattle and sheep. In the present study we investigated the possibility that H. somnus resists killing by bovine neutrophils, by causing the latter to undergo morphological changes consistent with apoptosis. Both serum-sensitive and serum-resistant strains of H. somnus enhanced bovine neutrophil chromatin condensation and shape change (i.e. zeiosis) in vitro, suggesting that the cells were undergoing apoptosis. Heat-killed or formalin-killed H. somnus had less effect than viable H. somnus. Chromatin margination of neutrophils was greater whenH. somnus was opsonized with adult bovine serum, which facilitates phagocytosis of the bacteria. H. somnus culture filtrates did not cause bovine neutrophil chromatin condensation. These findings suggest that direct contact with H. somnus is required for the maximal effect on bovine neutrophils. Apoptosis was confirmed by flow cytometry, using propidium iodide staining to detect DNA fragmentation. These findings suggest that H. somnus can evade killing by bovine neutrophils, in part, by inducing these cells to undergo apoptosis.

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Citations

Feb 17, 2001·Infection and Immunity·M J SylteC J Czuprynski
Sep 30, 2000·Clinical and Experimental Immunology·G ZyskH Heinz
Dec 11, 2008·Immunologic Research·Adam D Kennedy, Frank R DeLeo
Nov 28, 2006·Microbial Pathogenesis·Roger S GeertsemaLynette B Corbeil
Jan 26, 2008·Animal Health Research Reviews·Subramaniam SrikumaranAruna Ambagala
May 1, 2021·Vaccines·Rachael Lynda BellSara Louise Cosby
Dec 22, 1999·Trends in Pharmacological Sciences·C WardA G Rossi

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis