Apoptosis and expression of Fas/Fas ligand mRNA in bleomycin-induced pulmonary fibrosis in mice

American Journal of Respiratory Cell and Molecular Biology
Naoki HagimotoN Hara

Abstract

The incidence of apoptosis and the expression of Fas antigen (Fas)/Fas ligand (FasL) mRNA in bleomycin-induced pulmonary fibrosis in mice were examined. Male ICR mice were intratracheally instilled with bleomycin (5 U/kg of body weight). The controls were injected with sterile saline. The animals were anesthetized and killed at 1, 6, and 12 h, and 1, 3, 5, 7, 9, and 14 days after bleomycin instillation. We assessed the incidence of apoptosis in lung tissues by DNA fragmentation on agarose gel electrophoresis, terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end-labeling, and electron microscopy. The expression of Fas and FasL mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR). The localization of Fas mRNA was analyzed by in situ hybridization and that of FasL mRNA was analyzed by RT in situ PCR. The results showed that (1) a single instillation of bleomycin leads to the rapid appearance of apoptosis in bronchial and alveolar epithelial cells, which resolves within 1 day, and (2) apoptosis reappears on day 7 and continues for over 14 days after bleomycin instillation. This was accompanied with a progression of fibrosis. Corticosteroid administration completely blocked both apoptosis and ...Continue Reading

Citations

Aug 17, 2000·Springer Seminars in Immunopathology·C FonsecaC M Black
Apr 30, 1998·Hematology/oncology Clinics of North America·R J ArceciJ Pritchard
Apr 23, 2003·Free Radical Biology & Medicine·Arti ShuklaBrooke T Mossman
May 23, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·L M SutherlandA W Murray
Dec 17, 2008·Journal of Perinatology : Official Journal of the California Perinatal Association·B D Uhal, A Abdul-Hafez
Jan 9, 2009·Mucosal Immunology·M S Wilson, T A Wynn
Mar 1, 2003·Pathology International·Mohammed S Razzaque, Takashi Taguchi
Jun 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·William E LawsonTimothy S Blackwell
Sep 20, 2000·The Journal of Experimental Medicine·D W DavisD J McConkey
Apr 12, 2003·Critical Care Medicine·Thomas R MartinGustavo Matute-Bello
Feb 3, 2007·Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·Reinout A BemJob B M van Woensel
Jan 8, 2000·Fundamental & Clinical Pharmacology·A TrifilieffC Bertrand
Nov 26, 2004·Journal of Clinical Pathology·H MiyazakiY Nakanishi
Feb 16, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ognoon MungunsukhRegina M Day
Mar 29, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Amber L DegryseWilliam E Lawson
Sep 14, 2011·International Journal of Rheumatology·Stéphane Chabaud, Véronique J Moulin
May 29, 1998·American Journal of Respiratory and Critical Care Medicine·B T Mossman, A Churg
Jan 5, 2000·American Journal of Respiratory and Critical Care Medicine·S HashimotoH Nakajima
Aug 10, 2000·American Journal of Respiratory and Critical Care Medicine·K AoshibaA Nagai
Mar 20, 2001·American Journal of Respiratory and Critical Care Medicine·Y KitamuraH Nakajima
Mar 3, 2010·American Journal of Respiratory and Critical Care Medicine·Su Jin HwangDoo Hyun Chung
Dec 27, 2011·American Journal of Respiratory and Critical Care Medicine·Yoshinori AonoJo Rae Wright
Jul 18, 2002·American Journal of Respiratory and Critical Care Medicine·Ronald G CrystalRobert A Musson
Oct 6, 1997·American Journal of Respiratory Cell and Molecular Biology·N HagimotoN Hara
Oct 6, 1997·American Journal of Respiratory Cell and Molecular Biology·J L GoldbergB T Mossman
Oct 8, 1998·American Journal of Respiratory Cell and Molecular Biology·C BarazzoneP F Piguet
Nov 7, 1998·American Journal of Respiratory Cell and Molecular Biology·A DruilheM Pretolani
Dec 9, 1998·American Journal of Respiratory Cell and Molecular Biology·N HussainM J Kresch
Dec 31, 1998·American Journal of Respiratory Cell and Molecular Biology·K KuwanoN Hara
Aug 26, 1999·American Journal of Respiratory Cell and Molecular Biology·N HagimotoN Hara
Feb 5, 2000·American Journal of Respiratory Cell and Molecular Biology·K Kuwano, N Hara
Apr 27, 2000·American Journal of Respiratory Cell and Molecular Biology·A Mishra Martin WJ 2cnd
Mar 14, 2001·American Journal of Respiratory Cell and Molecular Biology·F TakahashiY Fukuchi
Oct 6, 2001·American Journal of Respiratory Cell and Molecular Biology·T BetsuyakuR M Senior
Jun 21, 2003·American Journal of Respiratory Cell and Molecular Biology·Masaki FujitaRobert J Mason
Jun 7, 2003·American Journal of Respiratory Cell and Molecular Biology·Randolph H HastingsLeonard J Deftos
Sep 16, 2003·American Journal of Respiratory Cell and Molecular Biology·Masahide MoriSeiji Hayashi
Nov 8, 2005·American Journal of Respiratory Cell and Molecular Biology·Stephen K FrankelDavid W H Riches
Sep 23, 2006·American Journal of Respiratory Cell and Molecular Biology·Regina Golan-GerstlRaphael Breuer
Jul 21, 2007·American Journal of Respiratory Cell and Molecular Biology·Celine A Beamer, Andrij Holian
Jul 1, 2008·American Journal of Respiratory Cell and Molecular Biology·Quanfu MaoMonique E De Paepe
Apr 18, 2009·American Journal of Respiratory Cell and Molecular Biology·Seung-Ick ChaDavid W H Riches

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

American Thoracic Association Journals

Discover the latest respiratory research published by the journals from the American Thoracic Society.

Related Papers

American Journal of Respiratory Cell and Molecular Biology
Naoki HagimotoN Hara
American Journal of Respiratory and Critical Care Medicine
K AoshibaA Nagai
American Journal of Respiratory Cell and Molecular Biology
Regina Golan-GerstlRaphael Breuer
© 2022 Meta ULC. All rights reserved