PMID: 7576871Jun 1, 1995

Apoptosis and heterophagy of medial edge epithelial cells of the secondary palatine shelves during fusion

Archives of Histology and Cytology
K TaniguchiY Uchiyama


Recent in vivo and in vitro studies have suggested that medial edge epithelial (MEE) cells covering the lateral palatine shelves do not undergo cell death, but migrate into the oral and nasal epithelium or transform into mesenchymal cells. We, therefore, reexamined the fate of MEE cells during palatal fusion in rat embryos by in situ 3' nick end labeling of dUTP (TUNEL), electron microscopy, and immunohisto/cytochemistry. TUNEL staining revealed positive nuclei in the medial edge epithelium immediately prior to contact, in epithelial triangles formed between the epithelial seam and nasal or oral epithelium, in epithelial pearls, and in mesenchymal tissue near the epithelium. However, these TUNEL-positive cells were rarely present in the epithelial seam. Electron microscopy revealed MEE cells showing nuclear chromatin condensation and cell shrinkage, and apoptotic bodies in the fusing epithelium; these often contained apoptotic body-like structures as heterophagosomes. By double staining using a laser scanning microscope, TUNEL-positive nuclei were co-localized with lysosomal cysteine proteinases, cathepsin B or L in MEE and mesenchymal cells adjacent to the epithelium. These results suggest that MEE cells undergo apoptosis duri...Continue Reading


Apr 1, 1999·Cell Biochemistry and Biophysics·L R RobertsG J Gores
Nov 11, 2011·Histochemistry and Cell Biology·Jeong-Oh ShinHan-Sung Jung
Jan 21, 2012·Histochemistry and Cell Biology·Jeong-Oh ShinHan-Sung Jung
Nov 12, 2002·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·Eva Andrea Holtgrave, Gisela Stoltenburg-Didinger
Jul 12, 2011·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·Yukio KatoriGen Murakami
Nov 4, 2009·Indian Journal of Plastic Surgery : Official Publication of the Association of Plastic Surgeons of India·Wenli YuKathy K H Svoboda
Feb 26, 2013·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·Antonius J M LuijsterburgChristl Vermeij-Keers
Jul 31, 2012·Mechanisms of Development·Wern-Joo SohnJae-Young Kim
Jun 5, 2002·Plastic and Reconstructive Surgery·Sadi ErfaniMichael T Longaker
Jul 17, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ali Nawshad
Jun 15, 2010·Birth Defects Research. Part C, Embryo Today : Reviews·Robert M Greene, M Michele Pisano
Nov 18, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Rulang JiangAndrew C Lidral
Feb 7, 2012·Journal of Cellular and Molecular Medicine·Tian MengBing Shi
Jul 30, 2020·Birth Defects Research·Yu JiChengji J Zhou
Oct 2, 1998·The American Journal of Physiology·B JonesG J Gores
Feb 14, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Akira NakajimaCharles F Shuler
Apr 12, 2003·Anatomical Science International·Masako FujinoKazuhiro Eto
Feb 24, 2009·Experimental Biology and Medicine·Wenli YuKathy K H Svoboda
Apr 28, 2004·Plastic and Reconstructive Surgery·Judith M GurleyLinda J Sandell
Jan 17, 2020·Genes·Brandi LantzHeather L Szabo-Rogers

Related Concepts

Structure of Papilla Incisiva of Mouth
Apoptosis, Intrinsic Pathway
Electron Microscopy
Ctsl protein, rat
Cathepsin L
Rats, Wistar
Cathepsin B-Like Proteinase

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.