Apoptosis and nutrition: Involvement of amino acid transport system in repression of hybridoma cell death.

Cytotechnology
F Franěk, K Chládková-Šrámková

Abstract

Mouse hybridoma cells cultured on the verge of starvation-induced apoptosis, i.e. in a medium diluted with saline, proved to serve as a sensitive screening system for apoptosis-suppressing activity of nutrient medium components. Conventional amino acid mixtures were found to suppress the starvation-induced apoptosis, whereas a vitamin mixture was ineffective. (Franěk F (1995) Biotechnol. Bioeng. 45: 86-90). Recent experiments showed that suppression of apoptosis, and concurrent resumption of growth, could be achieved by addition of single substances at millimolar concentrations. The set of active substances included certain coded L-amino acids (glycine, alanine, serine, threonine, proline, asparagine, glutamine, histidine), non-coded amino acids (β-alanine, taurine, 4-aminobutyric acid), and a non-metabolizable analogue (2-aminoisobutyric acid). This finding shows that some amino acids do not act solely as nutrients, but also as specific signal molecules. The specificity of the effect points to the involvement of adaptively regulated amino acid transport systems A and N in maintaining the balance between triggering and suppression of starvation-induced apoptosis.

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Citations

Jul 19, 2008·Biotechnology and Bioengineering·W ZhouJ Aunins
May 7, 2002·Biotechnology and Bioengineering·Laurent Simon, M Nazmul Karim
Mar 6, 1998·Trends in Biotechnology·A J Mastrangelo, M J Betenbaugh
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Jan 29, 2002·Archives of Biochemistry and Biophysics·Frantisek FranekTomás Eckschlager
Feb 10, 1999·Biotechnology and Bioengineering·J GoswamiD I Wang
Mar 22, 2014·Journal of the American Society of Nephrology : JASN·Akihiro ShimomuraHiromi Rakugi

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