Abstract
Mouse hybridoma cells cultured on the verge of starvation-induced apoptosis, i.e. in a medium diluted with saline, proved to serve as a sensitive screening system for apoptosis-suppressing activity of nutrient medium components. Conventional amino acid mixtures were found to suppress the starvation-induced apoptosis, whereas a vitamin mixture was ineffective. (Franěk F (1995) Biotechnol. Bioeng. 45: 86-90). Recent experiments showed that suppression of apoptosis, and concurrent resumption of growth, could be achieved by addition of single substances at millimolar concentrations. The set of active substances included certain coded L-amino acids (glycine, alanine, serine, threonine, proline, asparagine, glutamine, histidine), non-coded amino acids (β-alanine, taurine, 4-aminobutyric acid), and a non-metabolizable analogue (2-aminoisobutyric acid). This finding shows that some amino acids do not act solely as nutrients, but also as specific signal molecules. The specificity of the effect points to the involvement of adaptively regulated amino acid transport systems A and N in maintaining the balance between triggering and suppression of starvation-induced apoptosis.
References
Sep 1, 1991·Cytotechnology·F Franĕk, J Dolníková
Feb 1, 1992·Journal of Biotechnology·M C FlickingerS Boyce-Jacino
Jan 1, 1992·Cytotechnology·F FranĕkJ Dolníková
Apr 2, 1992·Nature·M C Raff
Dec 1, 1991·Molecular and Cellular Biology·S S GongC Basilico
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·P E NeimanG Loring
Feb 1, 1985·Journal of Cellular Physiology·M S KilbergT C Chiles
May 24, 1983·Biochimica Et Biophysica Acta·M A ShotwellD L Oxender
Dec 16, 1983·Journal of Immunological Methods·T Mosmann
Jan 1, 1994·Biotechnology Progress·T A BibilaJ G Aunins
Jan 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M S KilbergR O Laine
Jan 1, 1993·Immunology Letters·T Vomastek, F Franĕk
Aug 21, 1959·Science·H EAGLE
Nov 20, 1993·Biotechnology and Bioengineering·E C JoH M Moon
Jun 20, 1993·Biotechnology and Bioengineering·G W HillerH W Blanch
Sep 5, 1994·Biotechnology and Bioengineering·R P SinghA N Emery
Jan 5, 1995·Biotechnology and Bioengineering·F Franek
Citations
Jul 19, 2008·Biotechnology and Bioengineering·W ZhouJ Aunins
Apr 1, 1999·Biotechnology and Bioengineering·Y H KimE Suzuki
Jul 9, 1999·Biotechnology and Bioengineering·S MercilleB Massie
May 7, 2002·Biotechnology and Bioengineering·Laurent Simon, M Nazmul Karim
Jan 1, 1996·Cytotechnology·F Franěk, K Srámková
Sep 1, 1996·Immunology Letters·F Franĕk, K Srámková
Mar 6, 1998·Trends in Biotechnology·A J Mastrangelo, M J Betenbaugh
Mar 6, 1998·Journal of Biotechnology·M DoverskogL Häggström
Jul 1, 1997·Cytotechnology·S TeradaE Suzuki
Jan 1, 1997·Cytotechnology·E SuzukiJ C Reed
Nov 13, 2008·Cytotechnology·Y H KimE Suzuki
Nov 13, 2008·Cytotechnology·A Martial-GrosA Marc
Jan 1, 1997·Cytotechnology·F Franěk, K Srámková
Jan 29, 2002·Archives of Biochemistry and Biophysics·Frantisek FranekTomás Eckschlager
Jun 18, 2015·Biotechnology Progress·Abhishek J GuptaJan-Willem Boots
Nov 6, 2008·Journal of Biotechnology·Sun Ok Hwang, Gyun Min Lee
Feb 10, 1999·Biotechnology and Bioengineering·J GoswamiD I Wang
Apr 1, 1999·Biotechnology and Bioengineering·N H SimpsonM Al-Rubeai
Mar 22, 2014·Journal of the American Society of Nephrology : JASN·Akihiro ShimomuraHiromi Rakugi