Apoptosis as a Prognostic Factor in Colorectal Carcinoma: Comparison of TUNEL Method and Immunohistochemical Expression of Caspase-3

Applied Immunohistochemistry & Molecular Morphology : AIMM
Nenad KunacKatarina Vilović

Abstract

The development of colorectal cancer is known to be characterized by a sequence of events during which normal colonic epithelium gradually transforms to carcinoma, the adenoma-carcinoma sequence. Apoptosis plays an important role in the development and maintenance of tissue homeostasis. Currently, there is no agreement in the literature about the prognosis of apoptosis in colorectal cancer. The number of studies examining the expression of caspases in colorectal cancer is very limited, and they have not examined any correlation between expression and patient survival. This study included histologic samples from 179 patients diagnosed with colon cancer. We used the TdT-mediated X-dUTP nick end labeling method and caspase-3 labeling to identify the degree of apoptosis. Our results show that lower apoptotic index measured by TdT-mediated X-dUTP nick end labeling method and lower immnuhistochemical expression of caspase-3 is associated with shorter disease-free survival and overall survival. However, only apoptotic index is proven to be an independent survival indicator. The results of our study are consistent with the proposed models of carcinogenesis of colorectal cancer that emphasize resistance to apoptosis as a decisive factor...Continue Reading

References

Jun 1, 1990·Cell·E R Fearon, B Vogelstein
Apr 3, 1998·Surgery Today·K SugamuraN Kaibara
Apr 14, 1999·Cell Death and Differentiation·A G Porter, R U Jänicke
Dec 1, 1999·Cell Death and Differentiation·D W Nicholson
Jan 27, 2000·Cell·D Hanahan, R A Weinberg
May 15, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·L E JongesP J Kuppen
Mar 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Allen M Gown, Mark C Willingham
Dec 17, 2002·Critical Reviews in Oncology/hematology·J J KoornstraJ H Kleibeuker
Sep 23, 2003·Experimental Cell Research·Dunja LukovicDavid S Ucker
Jun 16, 2004·Cancer Metastasis Reviews·Lucia Malaguarnera
Aug 3, 2004·Nature Reviews. Cancer·Hitoshi Okada, Tak W Mak
Oct 14, 2004·Gut·A J M Watson
Feb 16, 2005·Biochemical Pharmacology·Magdalena HyzyAndrzej Skladanowski
Jan 10, 2006·Diseases of the Colon and Rectum·Marja HilskaPeter J Roberts
Sep 7, 2007·International Journal of Colorectal Disease·Javier de OcaAntoni Rafecas
Jun 21, 2011·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Sep 29, 2011·Journal of Experimental & Clinical Cancer Research : CR·Rebecca S Y Wong
Jan 11, 2013·Journal of Cellular and Molecular Medicine·Mayur V JainMarek Łos
Apr 3, 2013·Cold Spring Harbor Perspectives in Biology·David R McIlwainTak W Mak

❮ Previous
Next ❯

Citations

Jul 19, 2020·Microscopy Research and Technique·Sara SalucciElisabetta Falcieri
Mar 23, 2021·Frontiers in Cell and Developmental Biology·Xi-Min HuKun Xiong
Aug 26, 2021·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Weiling LengQinan Wu

❮ Previous
Next ❯

Software Mentioned

Statistical Package for Social Sciences

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.