Apoptosis-associated speck-like protein containing a caspase-1 recruitment domain (ASC) contributes to osteoblast differentiation and osteogenesis

Journal of Cellular Physiology
Suelen SartorettoWillian F Zambuzzi

Abstract

The role of apoptosis-associated speck-like protein containing a caspase-1 recruitment domain (ASC) in bone healing remains to be understood. To address this issue, we investigated the requirement of inflammasome-related genes in response to bone morphogenetic protein 7 (BMP7)-induced osteoblast differentiation in vitro. To validate the importance of ASC on osteogenesis, we subjected wild-type (WT) and ASC knockout C57BL/6 mice (ASC KO) to tibia defect to evaluate the bone healing process (up to 28 days). Our in vitro data showed that there is an involvement of ASC during BMP7-induced osteoblast differentiation, concomitant to osteogenic biomarker expression. Indeed, primary osteogenic cells from ASC KO presented a lower osteogenic profile than those obtained from WT mice. To validate this hypothesis, we evaluated the bone healing process of tibia defects on both WT and ASC KO mice genotypes and the ASC KO mice were not able to fully heal tibia defects up to 28 days, whereas WT tibia defects presented a higher bone de novo volume at this stage, evidencing ASC as an important molecule during osteogenic phenotype. In addition, we have shown a higher involvement of runt-related transcription factor 2 in WT sections during bone rep...Continue Reading

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Citations

Feb 26, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Renan C CuozzoMônica D Calasans-Maia
Jan 15, 2021·International Journal of Environmental Research and Public Health·Jhonathan R B NascimentoJosé A Calasans-Maia
Dec 10, 2020·Journal of Functional Biomaterials·Thiago Schneider Werner ViannaRafael Seabra Louro
May 16, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Suelen C SartorettoWillian F Zambuzzi
Jun 29, 2021·Frontiers in Immunology·Yang LiQianzhou Jiang
Aug 24, 2021·Tissue & Cell·Célio Júnior da C FernandesWillian F Zambuzzi
Dec 4, 2020·The Journal of Oral Implantology·Camila Saggioro de AlmeidaJosé Calasans-Maia de Albuquerque

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis