Apoptosis in cerebrum of macular mutant mouse

Acta Neuropathologica
Masaki OhnoMorimi Shimada

Abstract

Neuronal cell death in the brain of macular mutant mouse, a model of copper metabolism abnormality, has features of both apoptosis and necrosis. Apoptotic cells were morphologically identified by the terminal deoxynucleotidyl transferase nick end-labeling (TUNEL) method and electron microscopy. Numerous TUNEL-positive cells were identified in the cerebral cortex, hippocampus and thalamus of the hemizygotes after postnatal day 11. Ultramicroscopic studies confirmed that a number of cells had apoptotic features characterized by condensation and segregation of the nuclei. Furthermore, genomic DNA gel electrophoresis revealed a laddering pattern in the hemizygous brain. Starvation, which produced a low body weight in normal mice similar to that seen in the hemizygotes, did not result in an increase of TUNEL-positive cells. We also found that there was no increase of apoptotic cells in the brains of heterozygotes and copper-supplemented hemizygotes. Immunocytochemical analysis revealed that the distribution of copper/zinc superoxide dismutase-containing cells differed from that of TUNEL-positive cells. These findings suggest that copper deficiency is a key factor triggering apoptosis in the brain of macular mutant mouse through a me...Continue Reading

Citations

Jun 1, 2005·Molecular Genetics and Metabolism·Po-Ching LiuStephen G Kaler
Jun 24, 2008·Journal of the American Academy of Dermatology·Amy S Cheng, Susan J Bayliss
Jan 12, 2007·Molecular and Cellular Neurosciences·Rajaâ El MeskiniGabriele V Ronnett
Mar 5, 2016·Molecular Genetics and Metabolism Reports·Shoichi TakikitaYoshihiro Maruo
Nov 11, 2003·The Journal of Nutrition·Anna A Gybina, Joseph R Prohaska

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