PMID: 8908566Sep 1, 1996Paper

Apoptosis in chronic gastritis: evaluation of the gastric mucosa by DNA flow cytometry and the expression of the high molecular weight cytokeratin

Hepato-gastroenterology
Abdelfattah M AttallahF Ezzat

Abstract

A series of premalignant lesions, including chronic gastritis (CG), intestinal metaplasia (IM) and dysplasia are associated with gastric carcinogenesis. The present study aimed to define these precancerous gastric lesions further by the study of the cellular DNA using flow cytometry, and the expression of the high molecular weight (68 KDa) Cytokeratin "CK1" proposed as a marker for epithelial cells dying by apoptosis. Multiple antral biopsies from each of 92 cases with gastric dyspepsia were subjected for DNA content analysis using flow cytometry, and immunostaining using anti-CK1 monoclonal antibody. Chronic gastritis (CG) was present in 85 (92.4%) of cases, 14/85 (16.5%) cases showed chronic superficial gastritis (CSG), and 71/85 (83.5%) cases were chronic atrophic gastritis (CAG). Sixty two of the 85 (74.7%) cases with CG revealed variable degrees of activities. A hypodiploid "Sub-G1" peak was detected in 35 of 85 cases with CG. This peak was significantly higher in active chronic gastritis (ACG) than in the inactive (ICG) cases (p < 0.005). Proliferative activity of cases with CG was higher than in normal cases (p < 0.05) and in cases with ACG than in ICG (p < 0.05). Abnormal DNA-content (aneuploidy) was present in 16 (18.8...Continue Reading

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