Apoptosis in endothelial cells by cyclosporine

Transplantation Proceedings
E A HwangK C Mun

Abstract

The immunosuppressive drug cyclosporine (CsA) is a potent agent widely used after organ transplantations and to treat various autoimmune disorders. After using CsA, some patients suffer severe complications including renal and vascular toxicity, which are influenced by the degree of the endothelial damage. Several studies have demonstrated CsA treatment to directly induce apoptosis in several cell types. Thus, CsA may induce endothelial damage via activation of proapoptotic proteins. The present study was undertaken to investigate the effects of CsA on apoptosis of endothelial cells using human umbilical vein endothelial cells. Proliferation was measured by using the Cell Counting Assay Kit after cells were exposed to CsA (0 L, 10 L, 30 L, 50 L or 100 μg/mL). Apoptotic cells were identified by fluorescence microscopy of 4', 6-diamidino-2-phenylidole-stained nuclei. Western blot analysis was done for poly(ADP-ribose) polymerase (PARP), p27, p53 and caspase. Cell viability decreased dependent on the CsA concentration. CsA treatment group showed chromatin condensation and nuclear fragmentation. CsA produced a dose-dependent induction of p27 and reduction of procasapase-3. CsA treatment induced the degradation of 116-kDa PARP into ...Continue Reading

References

Mar 22, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·B LongoniF Mosca
Sep 19, 2006·Transplantation Proceedings·Y L OhE J Chang
Sep 19, 2006·Transplantation Proceedings·S Y HanK C Mun
Jan 26, 2011·Acta Dermato-venereologica·Christine D BouboukaAlexandra Katsarou
Jun 30, 2011·Advances in Experimental Medicine and Biology·Maria Ekoff, Gunnar Nilsson

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Citations

Jan 28, 2014·Annales de dermatologie et de vénéréologie·P PralongF Bérard
Apr 18, 2015·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Arkadiusz LubasStanisław Niemczyk
Mar 14, 2017·Bone Marrow Transplantation·E GavriilakiR A Brodsky
Jan 16, 2021·Pharmaceutics·Amandine GendronMariana Varna

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