Apoptosis in Hep2 cells treated with etoposide and colchicine

Cancer Detection and Prevention
Miroslav CervinkaEmil Rudolf

Abstract

When malignant cells undergo apoptosis, they exhibit many distinct patterns of behavior, with blebbing being one of the most spectacular and mysterious features. Despite huge advancements in our understanding of cell death, the mechanisms of apoptosis associated blebbing have not been elucidated. In order to verify the putative involvement of actin and tubulin in this process, Hep2 cells were treated with a combination of etoposide (10 microg/ml) and colchicine (0.2 microg/ml) for 24 h. Blebbing was analyzed using immunofluorescence staining of actin and tubulin, and the course of apoptosis was followed by time-lapse videomicroscopy, immunofluorescence detection of caspase-3 and cytokeratin fragment 18. The results indicate that microfilaments (actin) and not microtubules (tubulin) are involved in blebbing of Hep2 cells. Furthermore, despite the different mechanisms by which both chemicals act, their combined effects are not additive, but rather eliminate each other.

References

Mar 14, 1998·The Journal of Cell Biology·J C MillsR N Pittman
Apr 2, 1999·The European Journal of Neuroscience·A M GormanS Ceccatelli
Feb 26, 2000·Carcinogenesis·S W Lowe, A W Lin
Jul 25, 2000·Toxicology Letters·G Boos, H Stopper
Sep 1, 2000·Frontiers in Bioscience : a Journal and Virtual Library·E RudolfM Cervinka
Feb 19, 2002·Oncogene·Lena ThyrellDan Grandér
Feb 28, 2002·Cancer Chemotherapy and Pharmacology·Gary B RossonCynthia F Wright
Apr 26, 2002·Cell Death and Differentiation·R G Oshima
Jun 18, 2002·The Journal of Laboratory and Clinical Medicine·Can LiByung Kee Bang

❮ Previous
Next ❯

Citations

May 24, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Emil RudolfLadislava Schroterova
Sep 17, 2009·Lasers in Medical Science·Freek J van der MeerTon G van Leeuwen
Sep 24, 2005·Organic Letters·Timm GraeningHans-Günther Schmalz
Jun 8, 2018·Current Medicinal Chemistry·Tijana StankovićMilica Pešić
Oct 9, 2019·Journal of Medicinal Chemistry·Žiga SkokJanez Ilaš

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Cancer Imaging

Imaging techniques, including CT and MR, have become essential to tumor detection, diagnosis, and monitoring. Here is the latest research on cancer imaging.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis