Apoptosis in the uterus of mice with pregnancy loss

American Journal of Reproductive Immunology : AJRI
Shoshana SavionVladimir Toder

Abstract

The mechanisms mediating pregnancy loss induced by various agents are far from being understood. Thus, we investigated the possible involvement of one such mechanism, the apoptotic process, in pregnancy loss induced by lipopolysaccharide (LPS) or cyclophosphamide (CP) as well as the associated changes in the apoptosis-regulating gene products p53 and bcl-2. Pregnancy loss was induced by LPS or CP on days 9 or 12 of pregnancy, respectively. LPS- or CP-associated apoptosis was assessed by the TdT mediated dUTP-biotin nick end labeling (TUNEL) method as well as by DNA fragmentation analysis, while p53 or bcl-2 expression was evaluated by immunohistochemistry. Lipopolysaccharide treatment initiated a resorption process that was accompanied by the appearance of apoptotic cells in the uterus, which increased in number by 24 hr after treatment. Induction of pregnancy loss with CP resulted in the appearance of some apoptotic cells in the uterus, reaching a peak at 72 hr after treatment. DNA fragmentation analysis revealed a DNA ladder at 24 hr after LPS as well as 72 hr after CP treatment. Immunohistochemical analysis demonstrated a continuous p53 expression in the uterus of LPS- or CP-treated mice, which was somewhat elevated at the p...Continue Reading

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Citations

Jun 1, 2012·Journal of Developmental Origins of Health and Disease·T C HrubecS D Holladay
May 8, 2003·American Journal of Reproductive Immunology : AJRI·Shoshana SavionVladimir Toder
Feb 28, 2009·Human Reproduction·Yan FangXun Qu
Oct 14, 2010·Human Reproduction·Hong-Fei XiaJing-Pian Peng
Oct 26, 2010·Reproductive Biology and Endocrinology : RB&E·Gabriela F MeresmanJuan J Etchepareborda
Dec 12, 2013·Journal of Assisted Reproduction and Genetics·Belén LledoRafael Bernabeu
Jan 18, 2014·Molecular Biology Reports·L R FragaL Schuler-Faccini
Jan 9, 2003·American Journal of Reproductive Immunology : AJRI·V ToderA Torchinsky
Nov 12, 2014·Journal of Immunology Research·Xiaodan WangXiuhui Zhong
Mar 30, 2006·Reproductive Biomedicine Online·C B CoulamR S Jeyendran
Oct 28, 2015·Proceedings of the National Academy of Sciences of the United States of America·Chandrashekara KyathanahalliJennifer C Condon
Aug 20, 2008·Fertility and Sterility·Yogesh Kumar JaiswalMadan Mohan Chaturvedi
Jun 21, 2005·Scandinavian Journal of Immunology·A Zambon BertojaA C Zenclussen
May 13, 2009·The Australian & New Zealand Journal of Obstetrics & Gynaecology·Razieh Dehghani FirouzabadiNasim Tabibnejad
Nov 10, 2009·American Journal of Reproductive Immunology : AJRI·Shoshana SavionVladimir Toder
Jul 5, 2015·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Sang Ho YoonEun Chan Paik
Jul 5, 2016·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Ying ZhangWan-Jiang Zeng
Dec 21, 2004·Human Reproduction·Detlef PietrowskiClemens Tempfer
Jan 14, 2020·Environmental Science and Pollution Research International·Abd El-Fattah M El-ShershabyAbeer A Ahmed
Nov 7, 2017·Experimental and Therapeutic Medicine·Shuqin XiaAiming Wang
Dec 10, 2020·Theriogenology·Fariborz MoghadamMohammad Hossein Nasr Esfahani

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