Apoptosis induced by ardipusilloside III through BAD dephosphorylation and cleavage in human glioblastoma U251MG cells

Apoptosis : an International Journal on Programmed Cell Death
Hong LinPeng Wang

Abstract

Ardipusilloside III is a saponin newly isolated from Ardisia pusilla A.DC. Since saponins have exhibited broad anti-cancer and pro-apoptotic activity, we investigated the ability of ardipusilloside III to induce apoptosis in human glioblastoma U251MG cells, as well as the involvement of apoptotic signaling pathways. Ardipusilloside III markedly suppressed proliferation of U251MG cells in a time- and dose-dependent manner (P < 0.05, IC50 = 8.2 microg/ml), but did not affect the growth of primary cultures of human astrocytes. Ardipusilloside III-treated U251MG cells underwent typical apoptotic changes. Exposure to a low dose of ardipusilloside III provoked G2/M-phase cell cycle arrest, which preceded apoptosis characterized by the appearance of cells with sub-G1 DNA content. However, a higher dose of ardipusilloside III induced apoptosis without first causing cell cycle arrest. In addition, ardipusilloside III exposure resulted in time-dependent BAD dephosphorylation and cleavage as well as activation of caspase-8 and caspase-3. Therefore, both the intrinsic pathway of apoptosis, mediated by BAD dephosphorylation and cleavage, and the extrinisic pathway of apoptosis, mediated by caspase-8 and caspase-3 activation, were involved i...Continue Reading

References

Jan 1, 1990·Methods in Cell Biology·L Vindeløv, I J Christensen
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·D BaranesE R Kandel
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·S L SchendelJ C Reed
May 23, 1998·Annual Review of Immunology·D T Chao, S J Korsmeyer
Aug 28, 1998·Science·N A Thornberry, Y Lazebnik
Aug 28, 1998·Science·D R Green, J C Reed
Oct 26, 2000·Nature·M O Hengartner
Apr 5, 2001·Molecular and Cellular Biology·F CondorelliB Calabretta
Nov 28, 2001·Molecular Pharmacology·S C MastersH Fu
Jun 7, 2002·Molecular Cell·Yoshiyuki KonishiAzad Bonni
Jun 18, 2002·Journal of Neurochemistry·Thomas ZanderMichael T Heneka
Nov 15, 2002·Developmental Cell·Sandeep Robert DattaMichael E Greenberg
Jan 27, 2004·The Biochemical Journal·Yan ShouGary E Isom
Jul 3, 2004·The Journal of Biological Chemistry·So Young SeoJ Marie Hardwick
Sep 29, 2004·Cellular Signalling·Aleksandra Ellert-MiklaszewskaLiliana Konarska
Feb 17, 2005·European Journal of Pharmacology·An-Chin ChengMin-Hsiung Pan
Mar 8, 2005·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Xiang ZhangXi-Ling Wang
Jun 21, 2005·Annals of the New York Academy of Sciences·Chee-Fah ChiaChih-Hsiung Wu
Mar 7, 2006·The Journal of Nutritional Biochemistry·Jae In JungJung Han Yoon Park
Jul 18, 2006·Biochemical and Biophysical Research Communications·Simone GretheM Isabella Pörn-Ares
Jan 20, 2007·Acta Neuropathologica·Daisuke KitaHiroko Ohgaki
Feb 17, 2007·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Subbareddy MaddikaMarek Los
Jul 10, 2007·Acta Neuropathologica·David N LouisPaul Kleihues

❮ Previous
Next ❯

Citations

Jul 1, 2014·Fitoterapia·Mei ZhaoXinyou Liu
Jul 17, 2012·Pharmacology & Therapeutics·Sandra M Sancho-MartínezFrancisco J López-Hernández
Feb 22, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Lianqing LouJinhe Wang
Nov 10, 2015·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Siran YuZhizhen Zhang
Sep 24, 2009·Chemistry & Biodiversity·Hai-Feng TangGuang Cheng
Aug 12, 2015·Bioorganic & Medicinal Chemistry Letters·Dan-Qi LiChun-Fu Wu
Sep 15, 2012·Journal of Experimental & Clinical Cancer Research : CR·Nan YouKaishan Tao
Sep 3, 2013·Bioorganic & Medicinal Chemistry Letters·Xiao-Yang WangHai-Feng Tang
Oct 19, 2011·Toxicology Mechanisms and Methods·Elvira González de Mejía, Marco Vinicio Ramírez-Mares
May 30, 2015·Journal of Asian Natural Products Research·Hui LiHai-Feng Tang
Jun 29, 2016·Molecular Carcinogenesis·Nan YouLu Zheng
Mar 7, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yang LiuHaifeng Tang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Astrocytes & Neurodegeneration

Astrocytes are important for the health and function of the central nervous system. When these cells stop functioning properly, either through gain of function or loss of homeostatic controls, neurodegenerative diseases can occur. Here is the latest research on astrocytes and neurodegeneration.