Apoptosis induced by emodin is associated with alterations of intracellular acidification and reactive oxygen species in EC-109 cells

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
Q J WangX B Jing

Abstract

Emodin (1,3,8-trihydroxy-6-methylanthraquinone), a natural anthraquinone derivative found in several herbal medicines, is highly active in suppressing the proliferation of various tumor cells such as breast, hepatocellular, and lung cancer cells under in vitro conditions. The mechanism of emodin-induced apoptosis in esophagus carcinoma cells, EC-109, is not completely understood. In this study, EC-109 cells treated with emodin underwent rapid apoptosis as judged by morphological changes and flow cytometry analysis. The addition of emodin to EC-109 cells led to the inhibition of growth in a time- and dose-dependent manner. Fluorescence measurements of cells indicated that the intracellular pH (pHi) decreased significantly by 0.47-0.78 units. The results obtained from flow cytometry suggested that bursts of reactive oxygen species took place after the application of emodin. The present study indicates that emodin may be a strong anticancer drug against esophagus cancer cells by causing various early events leading to growth inhibition, including the production of reactive oxygen species and decrease of pHi, which may result in cellular apoptosis.

References

Jan 1, 1993·Archives of Biochemistry and Biophysics·M A Barry, A Eastman
Aug 1, 1997·Journal of Bioenergetics and Biomembranes·L D ShrodeS Grinstein
Feb 15, 2001·Cell Death and Differentiation·S Matsuyama, J C Reed
Apr 30, 2003·Japanese Journal of Cancer Research : Gann·Xubin JingToshikazu Hada
Oct 17, 2003·The Journal of Physiology·Julio F Turrens
Feb 28, 2004·Life Sciences·Den-En ShiehChun-Ching Lin
May 7, 2004·American Journal of Physiology. Cell Physiology·Elaine BeemMark S Segal
Apr 2, 2005·Methods in Cell Science : an Official Journal of the Society for in Vitro Biology·Cathrine NilssonKarin Ollinger
Dec 20, 2005·The Journal of Membrane Biology·R Schreiber
Feb 24, 2007·Archives of Biochemistry and Biophysics·Florian LangErich Gulbins
May 31, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Xu-Bin JingJu-Yu Cai

❮ Previous
Next ❯

Citations

Oct 28, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jin LiuTai Yang
Aug 13, 2011·Drug and Chemical Toxicology·Qiuxia HeXimin Wang
Sep 8, 2016·Journal of Agricultural and Food Chemistry·Christiane Gruber-DorningerFranz Berthiller
Sep 21, 2012·Oncology Reports·Jin-Xiang LiuSheng-Zhang Lin
Jul 27, 2021·Phytochemistry·Ruchi Badoni SemwalAlvaro Viljoen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis