Apoptosis induced by extracellular ATP in the mouse neuroblastoma cell line N1E-115: studies on involvement of P2 receptors and adenosine

Biochemical Pharmacology
S Mariëtte SchrierAd P IJzerman

Abstract

Adenosine triphosphate (ATP) can be released in large amounts from (damaged) cells, leading to locally high concentrations. In this study, we investigated the effect of such high concentrations of ATP on neuroblastoma cells. ATP (>or=30 microM) induced apoptosis in the mouse neuroblastoma cell line N1E-115. Activation of the ATP receptor P2X(7) is one of the routes via which ATP has been shown to induce apoptosis. Although the P2X(7) receptor was present in N1E-115 cells, both at the protein and mRNA level, studies with the P2X(7) receptor agonist benzoyl-benzoyl ATP showed that this receptor was not involved in ATP-induced apoptosis. It has been shown previously that adenosine induces apoptosis in N1E-115 cells after transport inside the cell. In this study, both dipyridamole, a nucleoside transport protein blocker, and uridine, a substrate for this transporter, were able to block ATP-induced apoptosis. This indicated that ATP had to be broken down to adenosine to induce apoptosis. The ecto-nucleotidase inhibitors 6-N,N-diethyl-beta-dibromomethylene-D-adenosine-5'-triphosphate (ARL67156) and alpha,beta-methylene adenosine 5'-diphosphate (AOPCP) commonly used to slow breakdown of ATP did not inhibit ATP breakdown appreciably, w...Continue Reading

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Citations

May 24, 2005·Apoptosis : an International Journal on Programmed Cell Death·D V KryskoK D'Herde
Jun 26, 2013·Purinergic Signalling·Geoffrey Burnstock, Francesco Di Virgilio
Oct 14, 2003·Biochemical Pharmacology·Audrone KalvelyteFeliksas F Bukauskas
Feb 7, 2009·Cell Death and Differentiation·E DecrockL Leybaert
Jul 19, 2011·Journal of Cellular and Molecular Medicine·Simona CosentinoMaria P Abbracchio
Jun 10, 2005·Journal of Biochemistry and Molecular Biology·Wentian SunChee Hong Tan
Aug 16, 2011·Biochimica Et Biophysica Acta·Antonio Rodríguez-SinovasDavid Garcia-Dorado
Dec 23, 2014·International Journal of Molecular Sciences·Kamila PuchałowiczVioletta Dziedziejko
Mar 17, 2006·Molecular Reproduction and Development·Francisco G Vázquez-CuevasRogelio O Arellano
Dec 15, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sarah J RaymentStephen P H Alexander
Jun 26, 2020·Frontiers in Pharmacology·Romain LaraShaun McNulty
Nov 8, 2005·World Journal of Gastroenterology : WJG·Ming-Xia WangBao-En Shan
May 1, 2021·International Journal of Molecular Sciences·Antonio Rodríguez-SinovasIgnacio Ferreira-González

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